Every lot is tried & tested in a relevant biological assay.
- Alomone Labs SR 48527 inhibits human NTS1 receptors expressed in CHO-K1 cells.Dose-response curve of hNTSR1 normalized inhibition by SR 48527 (#S-225). Cells were loaded with Fluo-8 dye, incubated with increasing concentrations of SR 48527, and stimulated with 3 nM neurotensin. Changes in intracellular Ca2+ following agonist application were detected as changes in maximum relative fluorescence (RLU) using FLIPRTETRA™. IC50 was calculated at 0.72 µM.
The two neurotensin receptors, NTR1 and NTR2, belong to the family of G-protein-coupled receptors1,2. The receptors are expressed in the brain and gastrointestinal tract where they play a role in pain transmission and intestinal motility.
SR 48527 (#S-225) is a highly pure, synthetic, and biologically active compound.