Overview
Type: Synthetic peptide
Form: Lyophilized powder
A Blocking peptide for Anti-STIM1 (extracellular) Antibody (#ACC-063) and Anti-STIM1 (extracellular)-ATTO Fluor-550 Antibody (#ACC-063-AO). Intended to be used as a negative control.
Applications: bl
For research purposes only, not for human use
Applications
Applications
Blocking
Western blot analysis of rat RBL basophilic leukemia (lanes 1 and 4), human HL-60 promyelocytic leukemia (lanes 2 and 5), and human Jurkat T-cell leukemia (lanes 3 and 6) cell lysates:1,2,3. Anti-STIM1 (extracellular) Antibody (#ACC-063), (1:1000).
4,5,6. Anti-STIM1 (extracellular) Antibody, preincubated with STIM1 (extracellular) Blocking Peptide (#BLP-CC063).
Expression of STIM1 in rat stomachImmunohistochemical staining of paraffin embedded rat stomach sections using Anti-STIM1 (extracellular) Antibody (#ACC-063), (1:100). STIM1 is expressed in the parietal cells of the gastric mucosa (arrows). Hematoxilin is used as the counterstain.
Expression of STIM1 in rat pancreasImmunohistochemical staining of rat paraffin-embedded pancreas sections using Anti-STIM1 (extracellular)-ATTO Fluor-550 Antibody (#ACC-063-AO), (1:20), (red). A. STIM1 staining is highly specific for endocrine cells in the Isle of Langerhans. B. Hoechst 33342 (blue) is used as the counterstain. C. Merged image of panels A and B.
Properties
Properties
Sequence
- CHSEDEKLSFEAVR, corresponding to amino acid residues 56-69 of human STIM1 (Accession Q13586).
Accession (Uniprot) Number Q13586
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Shipping and Ordering information
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