Cat #: BLP-CC063
Type: Synthetic peptide
Form: Lyophilized powder
A Blocking peptide for Anti-STIM1 (extracellular) Antibody (#ACC-063) and Anti-STIM1 (extracellular)-ATTO Fluor-550 Antibody (#ACC-063-AO). Intended to be used as a negative control.
For research purposes only, not for human use
- Western blot analysis of rat RBL basophilic leukemia (lanes 1 and 4), human HL-60 promyelocytic leukemia (lanes 2 and 5), and human Jurkat T-cell leukemia (lanes 3 and 6) cell lysates:
- Expression of STIM1 in rat stomachImmunohistochemical staining of paraffin embedded rat stomach sections using Anti-STIM1 (extracellular) Antibody (#ACC-063), (1:100). STIM1 is expressed in the parietal cells of the gastric mucosa (arrows). Hematoxilin is used as the counterstain.
- Expression of STIM1 in rat pancreasImmunohistochemical staining of rat paraffin-embedded pancreas sections using Anti-STIM1 (extracellular)-ATTO Fluor-550 Antibody (#ACC-063-AO), (1:20), (red). A. STIM1 staining is highly specific for endocrine cells in the Isle of Langerhans. B. Hoechst 33342 (blue) is used as the counterstain. C. Merged image of panels A and B.
- CHSEDEKLSFEAVR, corresponding to amino acid residues 56-69 of human STIM1 (Accession Q13586).
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
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