Overview
Type: Synthetic peptide
Form: Lyophilized powder
STIM1 (extracellular) Blocking Peptide (#BLP-CC063) is the original antigen used for immunization during Anti-STIM1 (extracellular) Antibody (#ACC-063) generation. The blocking peptide binds and ‘blocks’ Anti-STIM1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat RBL basophilic leukemia (lanes 1 and 4), human HL-60 promyelocytic leukemia (lanes 2 and 5), and human Jurkat T-cell leukemia (lanes 3 and 6) cell lysates:1,2,3. Anti-STIM1 (extracellular) Antibody (#ACC-063), (1:1000).
4,5,6. Anti-STIM1 (extracellular) Antibody, preincubated with STIM1 (extracellular) Blocking Peptide (#BLP-CC063).
Expression of STIM1 in rat stomachImmunohistochemical staining of paraffin embedded rat stomach sections using Anti-STIM1 (extracellular) Antibody (#ACC-063), (1:100). STIM1 is expressed in the parietal cells of the gastric mucosa (arrows). Hematoxilin is used as the counterstain.
Properties
Sequence
- CHSEDEKLSFEAVR, corresponding to amino acid residues 56-69 of human STIM1 (Accession Q13586).
Accession (Uniprot) Number Q13586
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
