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- The activity of this lot was confirmed by several assays: upon injection to Balb C mice, a value of about 4x107 MLD (median lethal doses) per mg protein has been obtained and typical Tetanus syndromes have been observed. The toxin also efficiently inhibited Ca2+-induced catecholamine release from permeabilized chromaffinergic neurons7.
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- Laemmli, V.K. (1970) Nature 227, 680.
- T. M. Cook, et al. (2001) Br. J. Anaesth. 87, 477.
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Tetanus toxin is a 150 kD protein produced by the gram positive bacteria Clostridium tetani under anaerobic condition1. Tetanus toxin is a hetrodimer of A-chain (50 kD) and B-chain (100 kD) linked by one disulfide bond2,3. The A-chain has a zinc endopeptidase activity while the B-chain has a role in toxin anchoring to neuronal membranes4.
Tetanus toxin spreads in the body through the lymphatic and vascular systems to the neuromuscular junction, internalized to peripheral neuron synapses, and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons to the CNS5. There, it inhibits neurotransmitter release by the specific degradation of the vesicle-secretory protein-Synaptobrevin6.
Tetanus Toxin (#T-150) is a highly pure, natural, and biologically active protein toxin.