Overview
Type: Synthetic peptide
Form: Lyophilized Powder
TMEM119 (extracellular) Blocking Peptide (#BLP-NR175) is the original antigen used for immunization during Anti-TMEM119 (extracellular) Antibody (#ANR-175) generation. The blocking peptide binds and ‘blocks’ Anti-TMEM119 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):1, 2. Anti-TMEM119 (extracellular) Antibody (#ANR-175), (1:200).
3, 4. Anti-TMEM119 (extracellular) Antibody, preincubated with TMEM119 (extracellular) Blocking Peptide (#BLP-NR175).
Western blot analysis mouse BV-2 microglia cell lysate:1. Anti-TMEM119 (extracellular) Antibody (#ANR-175), (1:200).
2. Anti-TMEM119 (extracellular) Antibody, preincubated with TMEM119 (extracellular) Blocking Peptide (#BLP-NR175).
Properties
Sequence
- Peptide (C)GTPAFSPTPERPQPT, corresponding to amino acid residues 53 - 67 of mouse TMEM119 (Accession Q8R138).
Accession (Uniprot) Number Q8R138
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
