Overview
Type: Synthetic peptide
Form: Lyophilized powder
TrkC (extracellular) Blocking Peptide (#BLP-NT020) is the original antigen used for immunization during Anti-TrkC (extracellular) Antibody (#ANT-020) generation. The blocking peptide binds and ‘blocks’ Anti-TrkC (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of HEK-TrkC transfected cells (lanes 1 and 3) and HEK untransfected cells (lanes 2 and 4) lysates:1,2. Anti-TrkC (extracellular) Antibody (#ANT-020), (1:400).
3,4. Anti-TrkC (extracellular) Antibody, preincubated with TrkC (extracellular) Blocking Peptide (#BLP-NT020).
Expression of TrkC in rat dorsal root gangliaImmunohistochemical staining of rat dorsal root ganglia (DRG) frozen sections using Anti-TrkC (extracellular) Antibody (#ANT-020), (1:100). A. TrkC labeling (red) appears in cell bodies. Note that the nerve fibers are not stained. B. Nuclear staining (blue) was visualized using Hoechst 33342. C. Merge of A and B.
Properties
Sequence
- (C)GHFLKEPFPESTD, corresponding to amino acid residues 393-405 of rat TrkC (Accession Q03351).
Accession (Uniprot) Number Q03351
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
