TrkC (extracellular) Blocking Peptide (#BLP-NT020) is the original antigen used for immunization during Anti-TrkC (extracellular) Antibody (#ANT-020) generation. The blocking peptide binds and ‘blocks’ Anti-TrkC (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of HEK-TrkC transfected cells (lanes 1 and 3) and HEK untransfected cells (lanes 2 and 4) lysates:
- Expression of TrkC in rat dorsal root gangliaImmunohistochemical staining of rat dorsal root ganglia (DRG) frozen sections using Anti-TrkC (extracellular) Antibody (#ANT-020), (1:100). A. TrkC labeling (red) appears in cell bodies. Note that the nerve fibers are not stained. B. Nuclear staining (blue) was visualized using Hoechst 33342. C. Merge of A and B.
- (C)GHFLKEPFPESTD, corresponding to amino acid residues 393-405 of rat TrkC (Accession Q03351).