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Certificate of Analysis

TRPA1 Channel Basic Research Pack

All You Need for TRPA1 Channel Research

Cat #: ESB-302
Size: 4 Vials
Form: Lyophilized

Antibodies

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-TRPA1 (extracellular) Antibody Cat #: ACC-037

Size: 0.2 ml Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, IP, LCI, WB Reactivity: H, M, R
Immunogen
Peptide (C)NSTGIINETSDHSE, corresponding to amino acid residues 747-760 of human TRPA1 (Accession O75762). 1st extracellular loop.
Homology
Bovine - identical; canis - 13/14 amino acid residues identical; rat - 12/14 amino acid residues identical; mouse - 9/14 amino acid residues identical.

Pharmacological Reagents

Ligustilide Cat #: L-170

Size: 25 mg Source: Natural MW: 190.2 Purity: >95%
Origin
Angelica sinensis.
Effective concentration
10-200 µM.
Activity
Ligustilide activates TRPA1 with an EC50 of 44 µM1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Up 100 mM in DMSO and 50 mM in Ethanol. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

CGP-37849 Cat #: C-325

Size: 10 mg Source: Synthetic MW: 209.14 Purity: >98%
Effective concentration
10-100 nM.
Activity
CGP-37849 is a potent and selective antagonist of NMDA receptors, inhibiting the binding of selective NMDA ligand [3H]-CPP to rat brain NMDA with Ki of 35 nM. Orally bioavailable anticonvulsant in vivo1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at +4°C.
Reconstitution
Up to 50 mM in water. May require gentle warming. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Store at -20°C. It is recommended to prepare solutions afresh on the day of use, or aliquot stock solutions stored at -20°C to prevent repeated thawing.

HC-030031 Cat #: H-105

Size: 100 mg Source: Synthetic MW: 355.4 Purity: >99%
Effective concentration
1-100 µM.
Activity
HC-030031 has an IC50 of 5 μM for antagonizing formalin-evoked Ca2+ influx1. It blocks TRPA1 channels.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Up to 100 mM in DMSO. Centrifuge all product preparations before use (5,000 x g for 1 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

For more information, please refer to individual certificate of analysis for each product.

For research purposes only, not for human use
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