Overview
Type: Synthetic peptide
Form: Lyophilized powder
TRPM7 Blocking Peptide (#BLP-CC047) is the original antigen used for immunization during Anti-TRPM7 Antibody (#ACC-047) generation. The blocking peptide binds and ‘blocks’ Anti-TRPM7 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat GH3 pituitary tumor cell line (lanes 1 and 2) and mouse brain (lanes 3 and 4) lysates:1,3. Anti-TRPM7 Antibody (#ACC-047), (1:200).
2,4. Anti-TRPM7 Antibody, preincubated with TRPM7 Blocking Peptide (#BLP-CC047).
Expression of TRPM7 in rat pituitary glandImmunohistochemical staining of rat hypophysis paraffin embedded transversal sections using Anti-TRPM7 Antibody (#ACC-047), (1:50). TRPM7 is stained in both the posterior part or neurohypophysis (N) and the anterior part or adenohypophysis (A). Note that stain is highly specific for andocrine cells in the adenohypophysis. Color reaction was obtained with SuperPicture HRP-conjugated polymer (Zymed) followed by DAB. Hematoxilin is used as the Counterstain.
Properties
Sequence
- CKRRKKDKTSDGPKLFLTEE, corresponding to amino acid residues 1146-1165 of human TRPM7 (Accession Q96QT4).
Accession (Uniprot) Number Q96QT4
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
