Overview
Type: Synthetic peptide
Form: Lyophilized powder
TRPML3/Mucolipin 3 Blocking Peptide (#BLP-CC083) is the original antigen used for immunization during Anti-TRPML3 (Mucolipin 3) Antibody (#ACC-083) generation. The blocking peptide binds and ‘blocks’ Anti-TRPML3/Mucolipin 3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain (lanes 1 and 4), kidney (lanes 2 and 5) and pancreas (lanes 3 and 6):1,2,3. Anti-TRPML3 (Mucolipin 3) Antibody (#ACC-083), (1:200).
4,5,6. Anti-TRPML3 (Mucolipin 3) Antibody, preincubated with TRPML3/Mucolipin 3 Blocking Peptide (#BLP-CC083).
Expression of Mucolipin 3 in mouse brain stemImmunohistochemical staining of TRPML3 in mouse brain stem using Anti-TRPML3 (Mucolipin 3) Antibody (#ACC-083). A. TRPML3 (green) appears in neurons (arrows) in the area of the dorsal cochlear nucleus. B. Staining of the same section with mouse anti-parvalbumin (red) reveals that the TRPML3 appears in a subset of neurons in this nucleus. DAPI is used as the counterstain (blue).
Properties
Sequence
- CKDLPNSGKYRLEDD, corresponding to amino acid residues 528-542 of mouse TRPML3 (Accession Q8R4F0).
Accession (Uniprot) Number Q8R4F0
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
