TRPML3/Mucolipin 3 Blocking Peptide (#BLP-CC083) is the original antigen used for immunization during Anti-TRPML3 (Mucolipin 3) Antibody (#ACC-083) generation. The blocking peptide binds and ‘blocks’ Anti-TRPML3/Mucolipin 3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain (lanes 1 and 4), kidney (lanes 2 and 5) and pancreas (lanes 3 and 6):
- Expression of Mucolipin 3 in mouse brain stemImmunohistochemical staining of TRPML3 in mouse brain stem using Anti-TRPML3 (Mucolipin 3) Antibody (#ACC-083). A. TRPML3 (green) appears in neurons (arrows) in the area of the dorsal cochlear nucleus. B. Staining of the same section with mouse anti-parvalbumin (red) reveals that the TRPML3 appears in a subset of neurons in this nucleus. DAPI is used as the counterstain (blue).
- CKDLPNSGKYRLEDD, corresponding to amino acid residues 528-542 of mouse TRPML3 (Accession Q8R4F0).