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Certificate of Analysis

TRPV1 Channel Premium Research Pack

All You Need for TRPV1 Channel Research

Cat #: ESP-300
Size: 6 Vials
Form: Lyophilized

Antibodies

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-TRPV1 (VR1) Antibody Cat #: ACC-030

Size: 0.2 ml Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)EDAEVFKDSMVPGEK, corresponding to amino acid residues 824-838 of rat TRPV1 (Accession O35433). Intracellular, C-terminus.
Homology
Mouse, guinea pig - 14/15 amino acid residues identical; human - 10/15 amino acid residues identical.

Guinea pig Anti-TRPV1 (VR1) Antibody Cat #: AGP-118

Size: 0.2 ml Host: Guinea pig Type: Polyclonal Application: IF, IH, WB Reactivity: R
Immunogen
Peptide (C)EDAEVFKDSMVPGEK, corresponding to amino acid residues 824-838 of rat TRPV1 (Accession O35433). Intracellular, C-terminus.
Homology
Mouse - 14/15 amino acid residues identical; human - 10/15 amino acid residues identical.

Anti-Rat TRPV1 (VR1) (extracellular) Antibody Cat #: ACC-029

Size: 0.2 ml Host: Rabbit Type: Polyclonal Application: IC, IF, IH, LCI, WB Reactivity: R
Immunogen
Peptide (C)NSLPMESTPHK*SRGS, corresponding to amino acid residues 605-619 of rat TRPV1 with replacement of cysteine 616 (C616) with serine (*S) (Accession O35433). 3rd extracellular loop.
Homology
Specific to rat TRPV1.

Pharmacological Reagents

Capsaicin Cat #: C-125

Size: 250 mg Source: Natural (from Capsicum sp.) MW: 305.4 Purity: >98% (HPLC)
Effective concentration
0.1-10 μM.
Activity
Capsaicin is one of the most powerful chemical agonists of the TRPV1 (transient receptor potential vanilloid) channel. It is a small lipophilic molecule derived from hot chili peppers that acts on sensory neurons by opening TRPV1, a principal transduction channel for nociception1. Capsaicin acts directly on TRPV12, with half-maximal effective concentration of 711.9 nM in Xenopus oocytes3 and 0.29 μM in HEK293 cells4.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Ethanol. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to one week at 4°C or six months at -20°C.

A-889425 Cat #: A-195

Size: 10 mg Source: Synthetic MW: 425.4 Purity: >98% (HPLC)
Effective concentration
0.5 nM - 50 µM.
Activity
A-889425 blocks TRPV1 channels with an IC50 of 335 nM (rat) and 34 nM (human)1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
DMSO up to 100 mM. Centrifuge all product preparations before use (10000 x g for 1 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

SB-366791 Cat #: S-180

Size: 25 mg Source: Synthetic MW: 287.7 Purity: >99%
Effective concentration
5 nM - 50 µM.
Activity
SB-366791 is a TRPV1 channel antagonist1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
100 mM in DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

For more information, please refer to individual certificate of analysis for each product.

Note
A Guinea pig polyclonal antibody (#AGP-118) is included in this Research Pack. Please take into account when reacting with a secondary antibody.
For research purposes only, not for human use
Shipping and Ordering information

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