Cat #: BLP-TR006
Type: Synthetic peptide
Form: Lyophilized powder
A Blocking peptide for Anti-TSH Receptor (TSHR) (extracellular) Antibody (#ATR-006) and Anti-TSH Receptor (TSHR) (extracellular)-ATTO Fluor-488 Antibody (#ATR-006-AG). Intended to be used as a negative control.
For research purposes only, not for human use
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:1,2. Anti-TSH Receptor (TSHR) (extracellular) Antibody (#ATR-006), (1:200).
3,4. Anti-TSH Receptor (TSHR) (extracellular) Antibody, preincubated with TSH Receptor/TSHR (extracellular) Blocking Peptide (#BLP-TR006).
- Expression of TSH Receptor in mouse hippocampus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-TSH Receptor (TSHR) (extracellular) Antibody (#ATR-006), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the hippocampal CA3 region, showed immunoreactivity (green) in the pyramidal layer (P, arrows) and in mossy fiber terminal region (MF). B. Pre-incubation of the antibody with TSH Receptor (TSHR) (extracellular) Blocking Peptide (BLP-TR006), suppressed staining. Cell nuclei are stained with DAPI (blue).
- Expression of TSH receptor in mouse brainImmunohistochemical staining of TSHR in mouse brain sections using Anti-TSH Receptor (TSHR) (extracellular)-ATTO Fluor-488 Antibody (#ATR-006-AG), (1:60). A. In the cerebellum, TSHR staining (green) appears in the molecular layer (MOL) and purkinje (P) layer. B. In the hippocampus, staining appears in CA3 pyramidal layer (P), (arrows). Nuclei are stained using DAPI (blue).
- ETLQAFDSHYDYTVC, corresponding to amino acid residues 376-390 of rat TSHR (Accession P21463).
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
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