Certificate of Analysis

TTX-Sensitive NaV Channel Antibody Explorer Kit

A Screening Package of TTX-Sensitive NaV Channel Antibodies Economically Priced
Cat #: AK-224 32 Vials

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-SCN1A (NaV1.1) Antibody Cat #: ASC-001

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide (C)TASEHSREPSAAGRLSD, corresponding to amino acid residues 465-481 of rat NaV1.1 (Accession P04774). Intracellular loop between domains I and II.
Homology
Human - identical.

Guinea pig Anti-SCN1A (NaV1.1) Antibody Cat #: AGP-043

Size: 50 µl Host: Guinea pig Type: Polyclonal Application: WB Reactivity: H, M, R
Immunogen
Peptide (C)TASEHSREPSAAGRLSD, corresponding to amino acid residues 465-481 of rat NaV1.1 (Accession P04774). Intracellular loop between domains I and II.
Homology
Mouse, human - identical.

Anti-SCN2A (NaV1.2) Antibody Cat #: ASC-002

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide (C)ASAESRDFSGAGGIGVFSE, corresponding to amino acid residues 467-485 of rat NaV1.2 (Accession P04775). Intracellular loop between domains I and II.
Homology
Human - identical.
NaV1.3 - 12/15 amino acid residues identical.

Guinea pig Anti-SCN2A (NaV1.2) Antibody Cat #: AGP-026

Size: 50 µl Host: Guinea pig Type: Polyclonal Application: IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)ASAESRDFSGAGGIGVFSE, corresponding to amino acid residues 467-485 of rat NaV1.2 (Accession P04775). Intracellular loop between domains I and II.
Homology
Human - identical.
NaV1.3 - 12/15 amino acid residues identical.

Anti-SCN3A (NaV1.3) Antibody Cat #: ASC-004

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide (C)HLEGNHRADGDRFP, corresponding to amino acid residues 511-524 rat NaV1.3 (Accession P08104). Intracellular, loop between domains I and II.
Homology
Mouse - 12/14 amino acid residues identical.

Anti-SCN3A (NaV1.3) (extracellular) Antibody Cat #: ASC-023

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, LCI, WB Reactivity: R
Immunogen
Peptide CSQWPPSDSAFE, corresponding to amino acid residues 277-288 of rat NaV1.3 (Accession P08104). 3rd extracellular loop, domain I.
Homology
Mouse, human – 11/12 amino acid residues identical.

Anti-SCN4A (NaV1.4) Antibody Cat #: ASC-020

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, WB Reactivity: H, M, R
Immunogen
Peptide EDKELKDNHILNHVG(C), corresponding to amino acid residues 877-891 of rat NaV1.4 (Accession P15390). Intracellular loop between domains II and III.
Homology
Mouse - 15/17 amino acid residues identical; human - 14/17 amino acid residues identical.

Anti-NaV1.6 (SCN8A) Antibody Cat #: ASC-009

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide CIANHTGVDIHRNGDFQKNG, corresponding to amino acid residues 1042-1061 of rat NaV1.6 (Accession O88420). Intracellular loop between domains II and III.
Homology
Mouse - identical; human - 19/20 amino acid residues identical.

Anti-NaV1.7 (SCN9A) Antibody Cat #: ASC-008

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)EFTSIGRSR IMGLSE, corresponding to amino acid residues 446-460 of rat NaV1.7 (Accession O08562). Intracellular loop between domains I and II.
Homology
Rabbit - 14/15 amino acid residues identical; human, mouse - 13/15 amino acid residues identical.

Guinea pig Anti-NaV1.7 (SCN9A) Antibody Cat #: AGP-057

Size: 50 µl Host: Guinea pig Type: Polyclonal Application: WB Reactivity: R
Immunogen
Peptide (C)EFTSIGRSR IMGLSE, corresponding to amino acid residues 446-460 of rat NaV1.7 (Accession O08562). Intracellular loop between domains I and II.
Homology
Human, mouse - 13/15 amino acid residues identical.

Anti-NaV1.7 (SCN9A) (extracellular) Antibody Cat #: ASC-027

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, LCI, WB Reactivity: R
Immunogen
Peptide (C)EKEGQTEYMDY(K), corresponding to amino acid residues 1533-1543 of rat NaV1.7 (Accession O08562). 1st extracellular loop, domain IV.
Homology
Mouse - 8/11 amino acid residues identical.
Human - Not recommended to use with human samples.

Anti-Pan NaV Antibody Cat #: ASC-003

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide TEEQKKYYNAMKKLGSKK(C), corresponding to amino acid residues 1501-1518 of rat NaV1.1 (Accession P04774). Intracellular loop between domains III and IV.
Homology
The epitope is identical in all isoforms of NaV1 in all vertebrates, and highly homologous in molluscan and insect voltage-gated Na+ channels.

Guinea pig Anti-Pan NaV Antibody Cat #: AGP-007

Size: 50 µl Host: Guinea pig Type: Polyclonal Application: WB Reactivity: H, M, R
Immunogen
Peptide TEEQKKYYNAMKKLGSKK(C), corresponding to amino acid residues 1501-1518 of rat NaV1.1 (Accession P04774). Intracellular loop between domains III and IV.
Homology
The epitope is identical in all isoforms of NaV1 in all vertebrates, and highly homologous in molluscan and insect voltage-gated Na+ channels.

Anti-SCN1B (NaVβ1) (extracellular) Antibody Cat #: ASC-041

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, LCI, WB Reactivity: M, R
Immunogen
Peptide CKRRSETTAETFTE, corresponding to amino acid residues 43-56 of rat β1 subunit of voltage-gated Na+ channels (Accession Q00954). Extracellular, N-terminus.
Homology
Mouse - identical; human -13/14 amino acid residues identical.

Anti-SCN2B (NaVβ2) Antibody Cat #: ASC-007

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)KTEEEGKTDGEGNAEDGAK, corresponding to amino acid residues 197-215 of rat β2 subunit of voltage-gated Na+ channels (Accession P54900). Intracellular, C-terminus.
Homology
Human - 17/19 amino acid residues identical.

Anti-SCN4B (NaVβ4) (extracellular) Antibody Cat #: ASC-044

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide (C)KNDKSDPKVRVKDD, corresponding to amino acid residues 85-98 of rat β4 subunit of voltage-gated Na+ channels (Accession Q7M730). Extracellular, N-terminus.
Homology
Mouse - identical; human - 11/14 amino acid residues identical.

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

Note
Guinea pig polyclonal antibodies (#AGP-043, #AGP-026, #AGP-057 & #AGP-007) are included in this Explorer Kit. Please take into account when reacting with a secondary antibody.