Overview
Type: Synthetic peptide
Form: Lyophilized powder
Urotensin II Receptor Blocking Peptide (#BLP-ER003) is the original antigen used for immunization during Anti-Urotensin II Receptor Antibody (#AER-003) generation. The blocking peptide binds and ‘blocks’ Anti-Urotensin II Receptor primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat kidney membranes:1. Anti-Urotensin II Receptor Antibody (#AER-003), (1:200).
2. Anti-Urotensin II Receptor Antibody, preincubated with Urotensin II Receptor Blocking Peptide (#BLP-ER003).- Rat paraffin kidney section.
Properties
Sequence
- (C)RPLDTVQRSKGYRK, corresponding to amino acid residues 154-167 of rat URIIR (Accession P49684).
Accession (Uniprot) Number P49684
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
