Every lot is tried & tested in a relevant biological assay.
- Alomone Labs Verapamil HCl inhibits L-type Ca2+ currents in Xenopus oocytes.A. Time course of L-type channel (CaV1.2+α2δ1+β1a) activity before and during applications of 10 and100 μM Verapamil HCl (#V-100) and upon wash (Verapamil HCl application periods are indicated by the horizontal bars). Holding potential was -100 mV and currents were elicited every 10 seconds by 100 ms steps to -10 mV. X axis in minutes and Y axis in nA. B. Example current traces before and during applications of 10 and 100 μM Verapamil HCl (concentrations indicated are taken from the experiment described in A).
- Haller, H. (2008) Int. J. Clin. Pract. 62, 781.
- Hockerman, G. H. et al. (1997) Annu. Rev. Pharmacol. Toxicol. 37, 361.
- Zanchetti, A. et al. (1998) J. Hypertension 16, 1667.
- Schamroth, L. et al. (1972) Brit. Med. J. 1, 660.
- Lacinova, L. et al. (1995) J. Pharmacol. Exp. Ther. 274, 54.
- Huber, I.G. et al. (2004) J. Biol. Chem. 279, 55211.
L-type (CaV1), voltage-gated Ca2+ channels are plasma membrane protein complexes which allow the passage of Ca2+ ions into cells following depolarization of the membrane potential. L-type channels are widely expressed in cardiac and smooth muscle in which they control contraction and therefore were recognized as a therapeutic target for cardiovascular diseases1,2.
Verapamil HCl is a phenylalkylamine that acts as a L-type, voltage-gated Ca2+ channel blocker and is used in the clinic to treat hypertension2-4. Verapamil is also effective in treating arrythmias2. Verapamil block of L-type channels is dependent on the membrane potential. The IC50 values at a holding potential of -80 or -40 mV are 250 and 15.5 µM, respectively5. In Xenopus oocytes, Verapamil inhibits L-type currents in an activity dependent manner6.
Verapamil HCl (#V-100) is a highly pure, synthetic, and biologically active compound.