VPAC1/VIPR1 (extracellular) Blocking Peptide (#BLP-VR001) is the original antigen used for immunization during Anti-VPAC1 (VIPR1) (extracellular) Antibody (#AVR-001) generation. The blocking peptide binds and ‘blocks’ Anti-VPAC1/VIPR1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain lysate (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and human Jurkat T cell leukemia cell lysate (lanes 3 and 6):
- Expression of VPAC1 in rat amygdalaImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-VPAC1 (VIPR1) (extracellular) Antibody (#AVR-001), (1:100). A. VPAC1 staining (red) is apparent in basolateral amygdala neurons (horizontal arrow). B. Cell nuclei in the same section are stained with DAPI (Blue). C. Merge of the two images.
- (C)EEAQLENETIG(S)SK, corresponding to amino acid residues 52-65 of human VPAC1 (Accession P32241).