Overview
Type: Synthetic peptide
Form: Lyophilized powder
VPAC1/VIPR1 (extracellular) Blocking Peptide (#BLP-VR001) is the original antigen used for immunization during Anti-VPAC1 (VIPR1) (extracellular) Antibody (#AVR-001) generation. The blocking peptide binds and ‘blocks’ Anti-VPAC1/VIPR1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and human Jurkat T cell leukemia cell lysate (lanes 3 and 6):1-3. Anti-VPAC1 (VIPR1) (extracellular) Antibody (#AVR-001), (1:400).
4-6. Anti-VPAC1 (VIPR1) (extracellular) Antibody, preincubated with VPAC1/VIPR1 (extracellular) Blocking Peptide (#BLP-VR001).
Expression of VPAC1 in rat amygdalaImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-VPAC1 (VIPR1) (extracellular) Antibody (#AVR-001), (1:100). A. VPAC1 staining (red) is apparent in basolateral amygdala neurons (horizontal arrow). B. Cell nuclei in the same section are stained with DAPI (Blue). C. Merge of the two images.
Properties
Sequence
- (C)EEAQLENETIG(S)SK, corresponding to amino acid residues 52-65 of human VPAC1 (Accession P32241).
Accession (Uniprot) Number P32241
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
