[Y]6-Angiotensin II Analog

A Potent and Selective AT2R Agonist
  • New
Cat #: SPA-175
  • Lyophilized Powder
  • Bioassay Tested
  • Source Synthetic peptide.
    MW: 1072 Da.
    Purity: ≥98% (HPLC)
    Effective concentration 1-100 nM.
    Sequence DRVYIYPF.
    Structure
    Molecular formula C53H73N11O13.
    Activity Y6-Angiotensin Analog is an AT2R agonist1,2.
    References - Activity
    1. Magnani, F. et al. (2014) ACS Chem. Biol. 9, 1420.
    2. Ishiguro, S. et al. (2015) Cancer Biol. Ther. 16, 307.
    Storage before reconstitution Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Reconstitution Soluble in water. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage after reconstitution Up to four weeks at 4°C or three months at -20°C.
    Our bioassay
    Alomone Labs [Y]6-Angiotensin II Analog induces ERK1/2 (p42/44 MAPK) phosphorylation in IEC-6 cells.
    Serum-deprived cells were stimulated for 5 min with 0  nM, 1 nM, 10 nM and 100 nM [Y]6-Angiotensin II Analog (#SPA-175) or with 100 nM Angiotensin II (#GPA-100). Cell proteins were resolved by SDS-PAGE and probed with anti-phospo-ERK1/2.
    References - Scientific Background
    1. Magnani, F. et al. (2014) ACS Chem. Biol. 9, 1420.
    2. Ishiguro, S. et al. (2015) Cancer Biol. Ther. 16, 307.
    Scientific background

    Y6-Angiotensin II Analog is a potent and selective agonist of Angiotensin II type 2 receptor (AT2R)1. Studies show that it can be used as a safe and effective therapeutic tool for pancreatic ductal adenocarcinoma1.

    Local administration of Y6-Angiotensin II Analog causes AT2R gene expression in tumor cells and significantly attenuates the growth of fast growing murine pancreatic ductal adenocarcinoma tumors. This evidence may suggest that AT2R agonist therapy is an effective and useful procedure for pancreatic ductal adenocarcinoma treatment2.

    Y6-Angiotensin II Analog shows two distinct sets of proton resonances in aqueous solution, cis and trans conformations1. The cis isomer contains aromatic rings of Tyr6 and Phe8 stacked around the Pro7 ring, thus leading to a compact hydrophobic motif1.

    Angiotensin II type 2 receptor belongs to the superfamily of G-protein coupled receptors. The receptor mediates angiotensin II actions involved in vasodilation and anti-proliferation2.

    Target AT2 receptor
    Net Peptide Content: 100%

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    Last update: 22/11/2018

    [Y]6-Angiotensin II Analog (#SPA-175), a highly purified synthetic peptide.

    For research purposes only, not for human use