Overview
Type: Synthetic peptide
Form: Lyophilized Powder
ZIP14 (SLC39A14) Blocking Peptide (#BLP-ZT024) is the original antigen used for immunization during Anti-ZIP14 (SLC39A14) Antibody (#AZT-024) generation. The blocking peptide binds and ‘blocks’ Anti-ZIP14 (SLC39A14) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):1-2. Anti-ZIP14 (SLC39A14) Antibody (#AZT-024), (1:200).
3-4. Anti-ZIP14 (SLC39A14) Antibody, preincubated with ZIP14 (SLC39A14) Blocking Peptide (BLP-ZT024).
Western blot analysis of mouse heart membranes (lanes 1 and 3) and rat heart membranes (lanes 2 and 4):1-2. Anti-ZIP14 (SLC39A14) Antibody (#AZT-024), (1:200).
3-4. Anti-ZIP14 (SLC39A14) Antibody, preincubated with ZIP14 (SLC39A14) Blocking Peptide (BLP-ZT024).
Western blot analysis of human LNCaP prostate carcinoma cell line lysates (lanes 1 and 3) and human THP-1 monocytic leukemia cell line lysates (lanes 2 and 4):1-2. Anti-ZIP14 (SLC39A14) Antibody (#AZT-024), (1:500).
3-4. Anti-ZIP14 (SLC39A14) Antibody, preincubated with ZIP14 (SLC39A14) Blocking Peptide (BLP-ZT024).
Expression of ZIP14 in mouse striatum.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-ZIP14 (SLC39A14) Antibody (#AZT-024), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. ZIP14 immunoreactivity (green) appears in neuron profiles (arrows). B. Pre-incubation of the antibody with ZIP14 (SLC39A14) Blocking Peptide (BLP-ZT024), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of ZIP14 in mouse cerebellum.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-ZIP14 (SLC39A14) Antibody (#AZT-024), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. ZIP14 immunoreactivity (green) appears in neuron profiles (arrows) and diffusely in the molecular layer (MOL). B. Pre-incubation of the antibody with ZIP14 (SLC39A14) Blocking Peptide (BLP-ZT024), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide (C)KDQEEGVTEKLQNGD, corresponding to amino acid residues 264 - 278 of mouse SLC39A14 (Accession Q75N73).
Accession (Uniprot) Number Q75N73
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
