ZIP6/SLC39A6 (extracellular) Blocking Peptide (#BLP-ZT006) is the original antigen used for immunization during Anti-ZIP6/SLC39A6 (extracellular) Antibody (#AZT-006) generation. The blocking peptide binds and ‘blocks’ Anti-ZIP6/SLC39A6 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
- Western blot analysis of human SH-SY5Y (lanes 1 and 3) and human K562 chronic myelogenous leukemia (lanes 2 and 4) cell line lysates:
- Expression of ZIP6 in rat hypothalamusImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-ZIP6/SLC39A6 (extracellular) Antibody (#AZT-006), (1:200), followed by goat anti-rabbit-AlexaFluor-488. ZIP6 immunoreactivity (green) appears in the median eminence (ME, arrows). Cell nuclei are stained with DAPI (blue). 3V = Third ventricle.
- (C)ESVSEPRKSFMYSR, corresponding to amino acid residues 251-264 of mouse ZIP6 (Accession Q8C145).