Overview
Type: Synthetic peptide
Form: Lyophilized powder
ZIP6/SLC39A6 (extracellular) Blocking Peptide (#BLP-ZT006) is the original antigen used for immunization during Anti-ZIP6/SLC39A6 (extracellular) Antibody (#AZT-006) generation. The blocking peptide binds and ‘blocks’ Anti-ZIP6/SLC39A6 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:1,2. Anti-ZIP6/SLC39A6 (extracellular) Antibody (#AZT-006), (1:200).
3,4. Anti-ZIP6/SLC39A6 (extracellular) Antibody, preincubated with ZIP6/SLC39A6 (extracellular) Blocking Peptide (#BLP-ZT006).
Western blot analysis of human SH-SY5Y (lanes 1 and 3) and human K562 chronic myelogenous leukemia (lanes 2 and 4) cell line lysates:1,2. Anti-ZIP6/SLC39A6 (extracellular) Antibody (#AZT-006), (1:400).
3,4. Anti-ZIP6/SLC39A6 (extracellular) Antibody, preincubated with ZIP6/SLC39A6 (extracellular) Blocking Peptide (#BLP-ZT006).
Expression of ZIP6 in rat hypothalamusImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-ZIP6/SLC39A6 (extracellular) Antibody (#AZT-006), (1:200), followed by goat anti-rabbit-AlexaFluor-488. ZIP6 immunoreactivity (green) appears in the median eminence (ME, arrows). Cell nuclei are stained with DAPI (blue). 3V = Third ventricle.
Properties
Sequence
- (C)ESVSEPRKSFMYSR, corresponding to amino acid residues 251-264 of mouse ZIP6 (Accession Q8C145).
Accession (Uniprot) Number Q8C145
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
