Immuno-Oncology

Immuno-Oncology

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

71 Results
  • 1-EBIO
    Bioassay Tested
    1-EBIO
    A Potent Activator of KCa3.1 and KCa2 Channels
    • Product Code: E-150
    • Short Description: A Potent Activator of KCa3.1 and KCa2 Channels
    • CAS No.: 10045-45-1
    • MW: 162.19
  • 17-AAG
    Bioassay Tested
    17-AAG
    An Anticancer Antibiotic
    • Product Code: A-580
    • Short Description: An Anticancer Antibiotic
    • CAS No.: 75747-14-7
    • MW: 585.7
  • Anti-AXL (extracellular) Antibody
    Anti-AXL (extracellular) Antibody
    A Rabbit Polyclonal antibody to AXL (extracellular)
    • Product Code: ATR-032
    • Short Description: A Rabbit Polyclonal antibody to AXL (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: AXL Receptor Tyrosine Kinase, Tyrosine-Protein Kinase Receptor UFO, AXL Oncogene, Tyro7, UFO
  • Anti-AXL (extracellular)-FITC Antibody
    Anti-AXL (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to AXL (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ATR-032-F
    • Short Description: A Rabbit Polyclonal Antibody to AXL (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: AXL Receptor Tyrosine Kinase, Tyrosine-Protein Kinase Receptor UFO, AXL Oncogene, Tyro7, UFO, ARK
  • Anti-CART Antibody
    Anti-CART Antibody
    A Rabbit Polyclonal antibody to CART peptide
    • Product Code: ANT-047
    • Short Description: A Rabbit Polyclonal antibody to CART peptide
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Cocaine-And Amphetamine-Regulated Transcript Protein, CARTPT, CART Prepropeptide
  • Anti-CX3CR1 (extracellular) Antibody
    Anti-CX3CR1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to CX3CR1 Receptor
    • Product Code: ACR-058
    • Short Description: A Rabbit Polyclonal Antibody to CX3CR1 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Fractalkine Receptor, CX3C chemokine receptor 1, C-X3-C CKR-1, C-X3-C motif chemokine receptor 1, GPR13, CMKBRL1, GPRV28, V28, G-protein coupled receptor 13, CCRL1, Beta chemokine receptor-like 1
  • Anti-CX3CR1 (extracellular)-APC Antibody
    Anti-CX3CR1 (extracellular)-APC Antibody
    A Rabbit Polyclonal Antibody to CX3CR1 (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)
    • Product Code: ACR-058-APC
    • Short Description: A Rabbit Polyclonal Antibody to CX3CR1 (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)
    • Host: Rabbit
    • Label: APC
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Fractalkine Receptor, CX3C chemokine receptor 1, C-X3-C CKR-1, C-X3-C motif chemokine receptor 1, GPR13, CMKBRL1, GPRV28, V28, G-protein coupled receptor 13, CCRL1, Beta chemokine receptor-like 1
  • Anti-CX3CR1 (extracellular)-FITC Antibody
    Anti-CX3CR1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to CX3CR1 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ACR-058-F
    • Short Description: A Rabbit Polyclonal Antibody to CX3CR1 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Fractalkine Receptor, CX3C chemokine receptor 1, C-X3-C CKR-1, C-X3-C motif chemokine receptor 1, GPR13, CMKBRL1, GPRV28, V28, G-protein coupled receptor 13, CCRL1, Beta chemokine receptor-like 1
  • Anti-Human CX3CR1 (extracellular)-FITC Antibody
    Anti-Human CX3CR1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to CX3CR1 Conjugated to the Fluorescent Dye FITC
    • Product Code: ACR-059-F
    • Short Description: A Rabbit Polyclonal Antibody to CX3CR1 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human
    • Applications: Live cell imaging, Flow cytometry
    • Target: Fractalkine Receptor, CX3C chemokine receptor 1, C-X3-C CKR-1, C-X3-C motif chemokine receptor 1, GPR13, CMKBRL1, GPRV28, V28, G-protein coupled receptor 13, CCRL1, Beta chemokine receptor-like 1
  • Anti-IGF1 Antibody
    Anti-IGF1 Antibody
    A Rabbit Polyclonal antibody to IGF1
    • Product Code: ANT-046
    • Short Description: A Rabbit Polyclonal antibody to IGF1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Insulin Like Growth Factor 1, Insulin-Like Growth Factor I, IGF-I, Somatomedin-C
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