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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2044 Results
  • Anti-Adenosine A<sub>2A</sub> Receptor (extracellular)-APC Antibody
    Anti-Adenosine A2A Receptor (extracellular)-APC Antibody
    A Rabbit Polyclonal Antibody to Adenosine A2A Receptor Conjugated to the Allophycocyanin (APC) fluorophore
    • Product Code: AAR-008-APC
    • Short Description: A Rabbit Polyclonal Antibody to Adenosine A2A Receptor Conjugated to the Allophycocyanin (APC) fluorophore
    • Host: Rabbit
    • Reactivity: Rat, Mouse
    • Applications: Flow cytometry
    • Target: ADORA2A, A2AAR, Adenosine A2aR
  • human BDNF-ATTO Fluor-647N
    Bioassay Tested
    human BDNF-ATTO Fluor-647N
    A fluorescent recombinant human BDNF for highly sensitive detection of TrkB/p75NTR receptors, live-cell imaging, neuronal development, and synaptic plasticity studies
    • Product Code: B-250-FRN
    • Short Description: A fluorescent recombinant human BDNF for highly sensitive detection of TrkB/p75NTR receptors, live-cell imaging, neuronal development, and synaptic plasticity studies
    • Applications: Apoptosis assay, Cell-Based Assays, Ex vivo, In vivo, Survival assay, Direct flow cytometry, Fluorescence staining, Live cell imaging, Immunofluorescence, Western blot
    • MW: 14134 Da
  • α-Conotoxin LtIA
    Bioassay Tested
    α-Conotoxin LtIA
    A potent and selective antagonist of the α3/β2 nicotinic acetylcholine receptor (nAChR)
    • Product Code: STC-555
    • Short Description: A potent and selective antagonist of the α3/β2 nicotinic acetylcholine receptor (nAChR)
    • Applications: Electrophysiology
    • MW: 1600.9 Da
  • Tetrodotoxin citrate
    Bioassay Tested
    Tetrodotoxin citrate
    Tetrodotoxin citrate (#T-550) is a highly pure, natural, and biologically active compound.
    • Product Code: T-550
    • Short Description: Tetrodotoxin citrate (#T-550) is a highly pure, natural, and biologically active compound.
    • Applications: Electrophysiology
    • CAS No.: 18660-81-6
    • MW: 319.27
  • Human TrkC-Fc Chimera
    Bioassay Tested
    Human TrkC-Fc Chimera
    A recombinant human TrkC-Fc fusion protein that acts as an inhibitor of NT-3 activity, preventing its binding to native TrkC receptors
    • Product Code: RPC-004
    • Short Description: A recombinant human TrkC-Fc fusion protein that acts as an inhibitor of NT-3 activity, preventing its binding to native TrkC receptors
    • Applications: Cell Culture, Western blot
    • MW: ~100 kDa
  • Human GFRα1-Fc Chimera
    Bioassay Tested
    Human GFRα1-Fc Chimera
    A recombinant human GFRα1-Fc fusion protein that acts as an inhibitor of GDNF activity, preventing its binding to the native RET receptor complex
    • Product Code: RPC-003
    • Short Description: A recombinant human GFRα1-Fc fusion protein that acts as an inhibitor of GDNF activity, preventing its binding to the native RET receptor complex
    • Applications: Cell Culture, Western blot
    • MW: ~85 kDa
  • Human TrkB-Fc Chimera
    Bioassay Tested
    Human TrkB-Fc Chimera
    A recombinant human TrkB-Fc fusion protein that acts as an inhibitor of BDNF and NT-4 activity, preventing their binding to native TrkB receptors
    • Product Code: RPC-001
    • Short Description: A recombinant human TrkB-Fc fusion protein that acts as an inhibitor of BDNF and NT-4 activity, preventing their binding to native TrkB receptors
    • Applications: Cell Culture, Western blot
    • MW: ~120 kDa
  • Recombinant human Artemin protein
    Bioassay Tested
    Recombinant human Artemin protein
    A recombinant human neurotrophic factor that promotes the survival of dopaminergic and motor neurons, primarily via the GFRα3 and RET receptor complex
    • Product Code: RPA-100
    • Short Description: A recombinant human neurotrophic factor that promotes the survival of dopaminergic and motor neurons, primarily via the GFRα3 and RET receptor complex
    • Applications: Cell Culture, Western blot
    • MW: 23.9 kDa (dimer)
  • Recombinant human Persephin protein
    Bioassay Tested
    Recombinant human Persephin protein
    A recombinant human neurotrophic factor that promotes the survival of dopaminergic and motor neurons, primarily via the GFRα4 and RET receptor complex
    • Product Code: P-320
    • Short Description: A recombinant human neurotrophic factor that promotes the survival of dopaminergic and motor neurons, primarily via the GFRα4 and RET receptor complex
    • Applications: Proliferation assay, Neurite outgrowth assay
    • MW: 20.6 kDa (dimer)
  • Recombinant human proNGF (cleavage resistant) protein
    Bioassay Tested
    Recombinant human proNGF (cleavage resistant) protein
    A cleavage-resistant recombinant human proNGF that binds TrkA, p75NTR, and SORT1 receptors involved in both cell survival and apoptosis
    • Product Code: N-285
    • Short Description: A cleavage-resistant recombinant human proNGF that binds TrkA, p75NTR, and SORT1 receptors involved in both cell survival and apoptosis
    • Applications: Cell Culture, Survival assay, Neurite outgrowth assay
    • MW: 49.9 kDa (dimer)
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