Technical Service Guide: Labeled Products

Customer Information

First Name

Last Name

Company/Institution

Lab Position

Phone

Product Information

Catalog Number / Product Name

Lot Number

Purchase Date

Sales Order Number

Problem and Previous Experience

What is the specific problem you are experiencing?

Did this same vial of product work in the past? What were the results?

Did other lots of this product work in the past? Which lots? What were the results?

Samples and Reconstitution

At what solution did you reconstitute the lyophilized sample?

Did you aliquot the samples in single-use portions in fresh stock solution?

Did you use a fresh working solution?

Do you avoid multiple freeze-thaw cycles?

Did you properly store the reconstituted samples?

Staining and Detection

What method of detection was used?

What type of sample was stained (frozen tissue, paraffin-embedded tissue, tissue culture cells)?

What method of fixation was used (acetone, formalin, methanol)?

At what dilution(s) and concentration was the sample used and what was the diluent?

What pattern of staining was expected and what was observed?

If performing immunofluorescence, was the filter used appropriate for the fluorochrome?

Did you use secondary conjugate?

What secondary conjugate was used (biotin, FITC, Rhodamine, Texas Red) and from which company?

Controls

What positive and/or negative controls were done and what were the results?

It is appreciated that if images can be attached.

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In order to process your request as soon as we can, the information below is compulsory:

result images
positive control
negative control
predict images

If you tried different experiment conditions, please specify conditions you used and provide the corresponding images.

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Technical Service Guide: Labeled Products