Multiplex Immunodetection

Multiplex Immunodetection

Maximize Targets, Minimize Cross-Reactivity

We offer in-house–produced and thoroughly tested antibodies conjugated to a variety of fluorophores, as well as extracellular-epitope reagents optimized for multiplex immunofluorescence, immunocolocalization, and high-parameter imaging. Guinea pig–derived antibodies are also available, providing additional species flexibility to help researchers build highly specific panels with minimal cross-reactivity. All antibodies are rigorously validated for applications including immunohistochemistry (IHC), immunocytochemistry (ICC), western blot (WB), and flow cytometry.

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FAQs

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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223 Results
  • Anti-5HT1B Receptor (HTR1B) (extracellular)-ATTO Fluor-488 Antibody
    Anti-5HT1B Receptor (HTR1B) (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 1B Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: ASR-022-AG
    • Short Description: A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 1B Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Immunofluorescence, Flow cytometry, Immunohistochemistry
    • Target: 5-Hydroxytryptamine receptor 1B, Serotonin receptor 1B, Serotonin 1D beta receptor, 5HT1D-beta, HTR1DB, HTR1D2
  • Anti-5HT2B Receptor (HTR2B) (extracellular)-ATTO Fluor-488 Antibody
    Anti-5HT2B Receptor (HTR2B) (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 2B Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: ASR-035-AG
    • Short Description: A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 2B Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry, Immunohistochemistry
    • Target: 5-Hydroxytryptamine receptor 2B, Serotonin receptor 2B, 5-HT-2B, 5-HT2F, Stomach fundus serotonin receptor
  • Anti-5HT3B Receptor (HTR3B) (extracellular)-ATTO Fluor-488 Antibody
    Anti-5HT3B Receptor (HTR3B) (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 3B Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: ASR-032-AG
    • Short Description: A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 3B Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Rat, Mouse
    • Applications: Immunofluorescence, Flow cytometry, Immunohistochemistry
    • Target: 5-Hydroxytryptamine receptor 3B, Serotonin receptor 3B
  • Anti-5HT4 Receptor (HTR4) (extracellular)-FITC Antibody
    Anti-5HT4 Receptor (HTR4) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 4 Conjugated to the Fluorescent Dye FITC
    • Product Code: ASR-036-F
    • Short Description: A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 4 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: 5-Hydroxytryptamine receptor 4, Serotonin receptor 4
  • Anti-5HT7 Receptor/HTR7 (extracellular)-FITC Antibody
    Anti-5HT7 Receptor/HTR7 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to 5HT7 receptor (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ASR-037-F
    • Short Description: A Rabbit Polyclonal Antibody to 5HT7 receptor (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: 5-Hydroxytryptamine Receptor 7, Serotonin Receptor 7, 5-HT7, 5-HT-7
  • Anti-ACE2 (extracellular)-FITC Antibody
    Anti-ACE2 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to ACE2 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: AAC-012-F
    • Short Description: A Rabbit Polyclonal Antibody to ACE2 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Angiotensin-Converting Enzyme 2, ACE-Related Carboxypeptidase, Angiotensin-converting enzyme homolog, ACEH, Metalloprotease MPROT15
  • Anti-ADAM10 (extracellular)-FITC Antibody
    Anti-ADAM10 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to ADAM10 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ANR-156-F
    • Short Description: A Rabbit Polyclonal Antibody to ADAM10 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Disintegrin And Metalloproteinase Domain-Containing Protein 10, ADAM Metallopeptidase Domain 10, CD156c
  • Anti-Adenosine A2A Receptor (extracellular)-FITC Antibody
    Anti-Adenosine A2A Receptor (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Adenosine A2A Receptor Conjugated to the Fluorescent Dye FITC
    • Product Code: AAR-008-F
    • Short Description: A Rabbit Polyclonal Antibody to Adenosine A2A Receptor Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: ADORA2A, A2AAR, Adenosine A2aR
  • Anti-Adenosine A<sub>2B</sub> Receptor (extracellular)-FITC Antibody
    Anti-Adenosine A2B Receptor (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Adenosine A2B Receptor Conjugated to the Fluorescent Dye FITC
    • Product Code: AAR-003-F
    • Short Description: A Rabbit Polyclonal Antibody to Adenosine A2B Receptor Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat
    • Applications: Flow cytometry
    • Target: ADORA2b, A2BAR, Adenosine A2bR
  • Anti-Adenosine A<sub>3</sub> Receptor (extracellular)-FITC Antibody
    Anti-Adenosine A3 Receptor (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Adenosine A3 Receptor (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: AAR-035-F
    • Short Description: A Rabbit Polyclonal Antibody to Adenosine A3 Receptor (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: ADORA3, A3AR, Adenosine Receptor A3
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