Technical Service Guide: Western Blotting Customer InformationFirst NameLast NamePhoneCompany/InstitutionEmailProduct InformationCatalog Number / Product NameProduct NameLot NumberPurchase DateProblem and Previous ExperienceWhat is the specific problem you are experiencing?What size bands were expected and what size bands were detected?Was the blot blank or was a dark background or non-specific bands seen?Did this same vial of product work in the past? What were the results?Did other lots of this product work in the past? Which lots? What were the results?Samples and ControlsFrom what species (animal) was the sample and what type of sample was used (cell lysate, tissue, purified protein, etc.)?What method of sample preparation was used?Was Alomone blocking peptide used as a negative control?What positive and/or negative controls were used, and what were the results?SDS-PageWhat percentage gel was used?What type of gel was used (Tris/glycine, Tris/acetate, etc.)?How much sample was loaded on the gel?At what temperature were the samples heated before loading (700C, 1000C, other)?Transfer and MembranesWhat type of transfer system was used (wet, semi-dry, other)?What were the transfer conditions (time, temperature, etc.)?What type of membrane was used (PVDF or nitrocellulose)?BlockingWhat were the blocking conditions (blocking solution, how long, what temperature. etc.)?Primary and Secondary AntibodiesHow was the primary antibody reconstituted and stored?At what dilution(s) was the primary antibody used and what was the diluent?What were the incubation conditions (time, temperature) for the primary antibody?What detection system was used?From which company was the secondary antibody obtained and what type of secondary was being used (HRP or AP conjugated, anti-rabbit, etc.)?At what dilution(s) was the secondary antibody used and what was the diluent?What were the incubation conditions (time, temperature) for the secondary antibody?In order to process your request as soon as we can, please attach result images below and make sure to indicate the molecular weight markers:Choose File If you tried different experiment conditions, please specify conditions you used and provide the corresponding images.Choose File Alomone Labs will process your data following our privacy policy. This will include sending you updates about us, our products, and related scientific information we believe would be of interest to you. You can manage your preferences or unsubscribe by clicking the link at the bottom of every email we send. Submit