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Detailed and Transparent Antibody Information – For You

You have a lot more information about antibody products than you might think.

“What was the immunogen used?”

“How do I reconstitute this?”

“What did you validate this in?”

We got a lot of questions like these and, in almost all cases, the answers are right there on the product page. We know that details are important to you, which is why we make the effort to put as much information on our product page as possible. That also means being completely transparent about things others vendors might not like to share. 

Immunogens are clearly displayed

Take antibodies’ immunogens, for example. When you ask some vendors for specific details about the immunogen used, quite often you’ll get a response along the lines of, “Sorry, that’s proprietary information.” Or you’ll get a vague and generally useless answer. We don’t agree with that. 

So, we give you the exact amino acid sequence used. Take our Anti-Human TRPV1 (extracellular) Antibody (#ACC-334). You already know it specifically targets the extracellular region of TRPV1, but let’s see what else there is. 

If you scroll a short way down to the Properties section, you’ll find an image that clearly shows the epitope, which is extracellular and on the first loop (Figures 1 and 2), it then gives you the amino acid sequence and some additional helpful information like an accession number that links to a UniProt entry. 

And because you have that sequence, you can easily deduce whether an antibody recognizes a particular protein in a species we haven’t tested by running a Blast to check for yourself. While the linked UniProt page lets you answer questions about a target protein’s expected molecular weight and any post-translational modifications like phosphorylation or glycosylation. 

Figure 1. The immunogen information for our Anti-Human TRPV1 (extracellular) Antibody (#ACC-334). In this section, you can see the immunogen used was a peptide with the sequence (C)RPVDGLPPFKMEKTGD, which corresponds to amino acid residues 456–471 of human TRPV1.
Figure 2. Where to easily find the Properties section on our data sheets.

Validation data is free to browse

You’ll find our testing, or validation, data at the very top of the page in the Applications section. If we stick with the Anti-Human TRPV1 (extracellular) Antibody (#ACC-334) example, you’ll see we validated this antibody in Western blot and flow cytometry across multiple cell lines (Figure 3)

Figure 3. Left: Western blot analysis of human MCF-7 breast adenocarcinoma cell line lysate (lanes 1 and 4), human LNCaP prostate adenocarcinoma cell line lysate (lanes 2 and 5), and human HMC3 microglia cell line lysate (lanes 3 and 6): 1-3. Anti-Human TRPV1 (extracellular) Antibody (#ACC-334), (1:500). 4-6. Anti-Human TRPV1 (extracellular) Antibody, preincubated with Human TRPV1 (extracellular) Blocking Peptide (BLP-CC334). Right: Cell surface detection of TRPV1 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cell line: Black = Cells. Red = Cells + goat-anti-rabbit-FITC. Green = Cells + Anti-Human TRPV1 (extracellular) Antibody (#ACC-334), (2.5μg) + goat-anti-rabbit-FITC.

Storage and handling data are also right there

Sometimes, it’s the simplest information we overlook, like what to reconstitute a reagent in, how to store it, or just the suggested working concentration. Because we make and test everything right here, in our labs, we make sure all of those, every day, essential bits of information are also right there. You’ll find information like this in the Properties section. You can see in the example below from the Anti-Human TRPV1 (extracellular) Antibody (#ACC-334) (Figure 4).

Just after the Properties section, you’ll see Scientific Background, which gives you information about the reagent, modes of action, or current research. And then, finally, there’s the Related Products section that lists different reagents from the same product group as well as products from different but related types you might find interesting or useful. 

Figure 4. The rest of the Properties section from Anti-Human TRPV1 (extracellular) Antibody (#ACC-334) shows all the useful information you need to start using this antibody right away or store it for later use. 

We’re here if you need more

But maybe there’s something else you need to know. Maybe, after looking through the Applications, the Properties, and the Scientific Background, you still need an answer to your question. In that case, just send us an email. As we said earlier, we design, make, and test everything in-house, so we have intimate knowledge of an antibody’s target and immediate family. We’re always happy to help you understand how something works or troubleshoot something that’s not working. 

Photo by Edwin Andrade.