Extracellular antibodies are ideal research tools for cell-surface proteins.
No permeabilization/fixation needed, because using extracellular antibodies allows you to work with live cells!
Benefits of live cell imaging: ✓ Monitor cell movement ✓ Study protein transport across the cell ✓ Investigate protein internalization
Immunocytochemical staining of live intact rat glioma C6 cells. A. Extracellular staining of cells using Anti-Calcium Sensing Receptor (extracellular) Antibody (#ACR-004), (1:50), followed by Alexa-555-conjugated goat-anti-rabbit secondary antibody. B. Nuclear fluorescence staining of cells using Hoechst 33342. C. Merged images of panels A and B.
Expression of TrkB in Living Rat PC12 Cells
Immunocytochemical staining of live intact rat pheochromocytoma PC12 cells. A. Extracellular staining of cells with Anti-TrkB (extracellular) Antibody (#ANT-019) (1:50), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells. C. Merge of the two images.
Expression of Kir2.1 in Living Rat RBL-2H3 Cells
Immunocytochemical staining of live intact rat RBL-2H3 basophilic leukemia cells. A. Extracellular staining of cells using Anti-Kir2.1 (extracellular) Antibody (#APC-159), (1:50), followed by goat-anti-rabbit-AlexaFluor-488 secondary antibody (green). B. Live view of the cells.
Expression of VPAC1 in Living Human HT-29 Cells
Immunocytochemical staining of live intact human HT-29 colorectal adenocarcinoma cells. A. Extracellular staining of live cells with Anti-VPAC1 (VIPR1) (extracellular) Antibody (#AVR-001), (1:25), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Cell nuclei were visualized using Hoechst 33342 (blue). C. Live view of the cells.
Expression of EphB1 in Living Rat PC12 Cells
Immunocytochemical staining of live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of cells with Anti-EphB1 (extracellular) Antibody (#AER-021), (1:100), followed by goat anti-rabbit-AlexaFluor- 594 (red). B. Live image of the cells. C. Merge of the two images.
Expression of Ghrelin Receptor in Living Human SH-SY5Y Cells
Immunocytochemical staining of intact living human neuroblastoma (SH-SY5Y) cells. A. Extracellular staining of cells with Anti-Ghrelin Receptor (GHSR) (extracellular) Antibody (#AGR-031), (red), (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. B. DAPI is used as the counterstain (blue). C. Merged images of A and B.
Expression of NET in Living Rat PC12 Cells
Immunocytochemical staining of live intact rat pheochromocytoma PC12 cells. Cells were stained with Anti-Noradrenaline Transporter (NET) (extracellular) Antibody (#AMT-002), (1:100), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). Cell nuclei (blue) were visualized using cell-permeable Hoechst 33342.
Expression of P2Y12 in Living Human U-87 MG Cells
Immunocytochemical staining of live intact human glioblastoma U-87 MG cells. A. Extracellular staining of cells using Guinea pig Anti-P2Y12 Receptor (extracellular) Antibody (#AGP-098), (1:100) followed by goat anti-guinea pig-AlexaFluor-594 secondary antibody (red). DAPI was used as the counterstain (blue). B. Live view of the cells merged with P2Y12 staining.
Expression of Kir4.1 in Living Human U-87 MG Cells
Immunocytochemical staining of live intact human glioblastoma U-87 MG cells. A. Extracellular staining of cells using Anti-Kir4.1 (KCNJ10) (extracellular) Antibody (#APC-165), (1:100) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live image of the cells. C. Merge of A and B.
Expression of SERT in Living Rat PC12 Cells
Immunocytochemical staining of live intact rat pheochromocytoma PC12 cells. A. Cells were stained with Anti-Serotonin Transporter (SERT) (extracellular) Antibody (#AMT-004), (1:100), followed by goat anti-rabbit-AlexaFluor- 594 secondary antibody (red). B. Cell nuclei were visualized using Hoechst 33342 (blue). C. Live view of the cells.
Expression of Magnesium Transporter NIPA2 in Living Rat C6 Cells
Immunocytochemical staining of rat brain glioma C6 cells. A. Extracellular staining of cells with Anti-NIPA2 (extracellular) Antibody (#ANT-142), (1:50), (red). B. Merged image of A with live view of cell.
Expression of Neuropilin-1 in Living Human U-87 MG Cells
Immunocytochemical staining of live intact human U-87 MG glioblastoma cells. A. Extracellular staining of cells using Anti-Neuropilin-1 (NRP1) (extracellular) Antibody (#ANR-063), (1:25). B. Live view of the cells.
Expression of CXCR5 in Living Human LNCaP Cells
Immunocytochemical staining of intact living human prostate carcinoma (LNCaP) cells. A. Extracellular staining of cells using Anti-Human CXCR5 (extracellular) Antibody (#ACR-015), (green), (1:25) merged with live view of the cells (magnitude X40). B. Extracellular staining of LNCaP cells merged with live view of the cells (magnitude X100).
Expression of TRPV6 in Living Human LNCaP Cells
Immunocytochemical staining of intact living human prostate carcinoma (LNCaP) cells. A. Extracellular staining of cells using Anti-HumanTRPV6 (extracellular) Antibody (#ACC-028), (1:50) followed by goat anti-rabbit-AlexaFluor-488 secondary antibody(green). B. Merge images of A with live view of the cells.
Expression of TRPV3 in Living Rat PC12 Cells
Immunocytochemical staining of intact living rat pheochromocytoma (PC12) cells. A. Extracellular staining of cells using Anti-TRPV3 (extracellular)-Biotin Antibody (#ACC-033-B), (1:50), (red). B. Merge of A with the live view of the cell.
Expression of GFRα4 in Living Rat PC12 Cells
Immunocytochemical staining of live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of cells with Anti-GFR alpha 4 (extracellular) Antibody (#ANT-024), (1:100), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Cell nuclei stained with the cell permeable dye Hoechst 33342 (blue). C. Merge of the two images.
Expression of DAT in Living Rat PC12 Cells
Immunocytochemical staining of live intact rat pheochromocytoma PC12 cells. A. Cells stained with Anti-Dopamine Transporter (DAT) (extracellular) Antibody(#AMT-003), (1:100), followed by goat anti-rabbit-AlexaFluor- 594 secondary antibody (red). B. Live view of the cells..
Expression of ASIC in Living Rat PC12 Cells
Immunocytochemical staining of live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of cells with Anti-pan ASIC (extracellular) Antibody(#ASC-031), (1:25), followed by goat anti-rabbit-AlexaFluor-488 secondary antibody (green). B. Live view of the cells. C. Merge of A and B.
Expression of HCN3 in Living Rat U-87 MG Cells
Immunocytochemical staining of intact living U-87 MG cells. A. Extracellular staining of cells using Anti-HCN3 (extracellular) Antibody (#APC-083), (1:25) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells. C. Extracellular staining merged with live view of the cells.
Expression of NaV1.3 in Living HEK-293 Transfected Cells
Immunocytochemical staining of intact living HEK-293 cells expressing rat NaV1.3. A. Extracellular staining of cells using Anti-SCN3A (NaV1.3) (extracellular) Antibody (#ASC-023), (red). B. Cells transfected with the empty vector show no NaV1.3 staining. Nuclear staining using DAPI as the counterstain (blue).
Expression of NHE-1 in Living Human MCF-7 Cells
Immunocytochemical staining of live intact human MCF-7 breast adenocarcinoma cells. A. Extracellular labeling of cells with Anti-Na+/H+ Exchanger 1 (NHE-1) (extracellular) Antibody(#ANX-010), (1:25), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells. C. Merge of the two pictures.
Expression of P2X4 in Living Rat PC12 Cells
Immunocytochemical staining of intact living PC12 cells. A. Extracellular staining of cells with Anti-P2X4 Receptor (extracellular) Antibody (#APR-024), (red), (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. B. Nuclear staining of cells using DAPI (blue). C. Merged images of A and B.
Expression of TrkA in Living Rat PC12 Cells
Immunocytochemical staining of live intact rat PC12 cells. A. Cells were stained with Anti-TrkA (extracellular) Antibody (#ANT-018) (1:50), followed by goat anti-rabbit-AlexaFluor-494 secondary antibody (red). B. Cell nuclei were visualized with the membrane-permeable DNA dye Hoechst 33342 (blue staining). C. Live view of the cells.
Expression of AT2 Receptor in Living Mouse 3T3-L1 Cells
Immunocytochemical staining of live intact rat pheochromocytoma PC12 cells. A. Extracellular staining of cells using Guinea pig Anti-GluR2 (GluA2) (extracellular) Antibody (#AGP-073), (1:50) followed by goat anti-guinea pig-AlexaFluor-594 secondary antibody (red). B. Live image of the cells. C. Merge of A and B.
Expression of KCNQ1 (KV7.1) in Living Human COLO-205 Cells
Immunocytochemical staining of live intact human COLO-205 colon adenocarcinoma cells. A. Extracellular staining of cells with Anti-KCNQ1 (extracellular) Antibody (#APC-168), (1:50), followed by goat anti-rabbit-AlexaFluor-488 secondary antibody (green). B. Live view of the cells. C. Merge of A and B.
Expression of Ret in Living Human MCF-7 Cells
Immunocytochemical staining of live intact human MCF-7 breast adenocarcinoma cells. A. Extracellular staining of cells with Anti-Ret (extracellular) Antibody (#ANT-025), (1:25), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live image of the cells. C. Merge of the two images.
Expression of Calcitonin Receptor in Living Rat PC12 Cells
Immunocytochemical staining of intact living rat pheochromocytoma (PC12) cells. A, C. Extracellular staining of cells using Anti-Calcitonin Receptor (extracellular) Antibody, (#ACR-053), (1:50). B, D. Merged images of A and C, each with its respective live view of the cells.
Expression of NaVβ1 in Living Rat PC12 Cells
Immunocytochemical staining of live and intact differentiated rat PC12 cells. PC12 differentiation was induced by Native mouse NGF 2.5S protein (>95%) (#N-100). A, C. Merge of NaVβ1 staining using Anti-SCN1B (NaVβ1) (extracellular) Antibody (#ASC-041), (1:50), (red) with live cell imaging. B, D. Merge of NaVβ1 staining (1:50), (red) with nuclear staining using DAPI as the counterstain.
Expression of EAAT2 in Living Human U-87 MG Cells
Immunocytochemical staining of live intact human U-87 MG glioblastoma cells. A. Extracellular staining of cells using Anti-EAAT2 (GLT-1) (extracellular) Antibody (#AGC-022), (1:100) followed by goat anti-rabbit-Alexa-Fluor-594 secondary antibody (red). B. Live image of the cells. C. Merge of A and B.
Expression of TRPV1 in Living Rat PC12 Cells
Immunocytochemical staining of intact living Pheochromocytoma (PC12) cells. A. Extracellular staining of cells using Anti-Rat TRPV1 (VR1) (extracellular)-ATTO Fluor-488 Antibody (#ACC-029-AG), (1:25), (green). B. live view of the cell. C. Merge images of A and B.
Expression of EphA1 in Living Human MCF-7 Cells
Immunocytochemical staining of live intact MCF-7 breast adenocarcinoma cells. Cells were stained with Anti-EphA1 (extracellular) Antibody (#AER-011), (1:25) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live image of the cells stained in A. C. Merge image of A and B.
Expression of Choline Transporter in Living Rat PC12 Cells
Immunocytochemical staining of live intact rat pheochromocytoma PC12 cells. A. Cells were stained with Anti-Choline Transporter (CHT) (extracellular) Antibody (#ACT-001), (1:100), followed by goat anti-rabbit-AlexaFluor- 594 secondary antibody (red). B. Live view of the cells. C. Merge of the two pictures.
Expression of KCa3.1 in Living Human LN-CaP Prostate Carcinoma Cells
Immunocytochemical staining of live intact human LN-CaP prostate carcinoma cells with Mouse Anti-KCNN4 (KCa3.1, SK4) (extracellular) Antibody (#ALM-051), (1:20), followed by goat-anti-mouse-DyLight-594 secondary antibody (red), (A). B. Live view of the cells. C. Merge of the two images.
Expression of RAMP1 in Living Rat PC12 Cells
Immunocytochemical staining of intact living rat pheochromocytoma (PC12) cells. A. Extracellular staining of cells using Anti-RAMP1 (extracellular) Antibody (#ARR-021), (1:50), (red). B. Live view of cells.
Expression of Parathyroid Hormone 1 Receptor in Living Rat U-87 MG Cells
Immunocytochemical staining of intact living rat U-87 MG cells. A. Extracellular staining of cells using Anti-PTH1R (extracellular) Antibody (#APR-051), (1:50), (green). B. Merge of A with live view of the cells.
Expression of NK1 Receptor in Living Rat PC12 Cells
Immunocytochemical staining of intact living rat pheochromocytoma (PC12) cells. A. Extracellular staining of cells using Anti-Neurokinin 1 Receptor (NK1R) (extracellular) Antibody (#ATR-001), (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Merge image of A and live view of the cells.
Expression of KV1.2 in Living Rat PC12 Cells
Immunocytochemical staining of live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of cells with Anti-KV1.2 (KCNA2) (extracellular) Antibody (#APC-162), (1:50), followed by goat anti-rabbit-AlexaFluor-488 secondary antibody (green). B. Live view of the cells. C. Merge of A and B.
Expression of P2X1 in Living Human SH-SYS5 Cells
Immunocytochemical staining of intact living human SH-SYS5 cells. A. Extracellular staining of cells with Anti-P2X1 Receptor (extracellular) Antibody (#APR-022), (1:50) followed by goat anti-rabbit-AlexaFluor-488 secondary antibody. B. Nuclear staining DAPI as the counterstain. C. Merged images of A and B.
Expression of NaV1.7 in Living Rat PC12 Cells
Immunocytochemical staining of intact living rat pheochromocytoma PC12 cells. A. Extracellular staining of cells using Anti-NaV1.7 (SCN9A) (extracellular) Antibody (#ASC-027), (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Merge image of A and live view of the cells.
Expression of mGluR4 in Living Rat PC12 Cells
Immunocytochemical staining of live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of cells using Anti-mGluR4 (extracellular) Antibody (#AGC-014), (1:50), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells. C. Merge of A and B. Nuclei are stained with Hoechst 33342 (blue).
Expression of EAAT1 in Living Human U-87 MG Cells
Immunocytochemical staining of live intact human U-87 MG glioblastoma cells. A. Extracellular staining of cells using Anti-EAAT1 (GLAST) (extracellular) Antibody (#AGC-021), (1:100) followed by goat anti-rabbit-Alexa-Fluor-594 secondary antibody (red). B. Live image of the cells. C. Merge of A and B.
Expression of SSTR5 in Living Human HT-29 Cells
Immunocytochemical staining of intact living human colorectal adenocarcinoma (HT-29) cells. A. Extracellular staining of cells with Anti-Somatostatin Receptor Type 5 (extracellular) Antibody (#ASR-005), (1:50), (red). B. Merged image of A with live view of cell.
Expression of nAChR α7 in Living Rat PC12 Cells
Immunocytochemical staining of live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of cells with Anti-Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular) Antibody(#ANC-007), (1:25), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells. C. Merge of A and B.
Expression of Adenylate Cyclase Type III in Living Rat U-87 MG Cells
Expression of KV1.3 in Living HEK 293 Transfected Cells
Immunocytochemical staining of live intact HEK 293 cells transfected with rat KV1.3 (panels A and C) or with empty vector (panels B and D) using Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101), (1:50). Staining (green) shows expression of KV1.3 in the transfected cells (A) but not in the cells transfected with empty vector (B). Panels C and D show the corresponding live image of the cells.
Expression of GABA(A) α1 Receptor in Living Rat PC12 Cells
Immunocytochemical staining of intact living rat pheochromocytoma PC12 cells. A. Extracellular staining of cells using Anti-GABA(A) α1 Receptor (extracellular) Antibody (#AGA-001), (1:100) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. B. Merge image of A and live view of the cells.
Expression of Adenosine A2B Receptor in Living Rat PC12 Cells
Immunocytochemical staining of intact living rat pheochromocytoma PC12 cells. A. Extracellular staining of cells using Anti-Adenosine A2B Receptor (extracellular) Antibody (#AAR-003), (1:100) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. B. Merge of A and live view of the cells.
Expression of Ephrin-A1 in Living Rat PC12 Cells
Immunocytochemical staining of live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of cells with Anti-Ephrin-A1 (extracellular) Antibody (#AER-031), (1:50), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells. C. Merge of A and B.
Expression of Glutamate Receptor δ1 in Living Rat PC12 Cells
Immunocytochemical staining of live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of cells with Anti-GRID1 (extracellular) Antibody (#AGC-038), (1:50), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells. C. Merge of A and B.
Expression of SSTR2 in Living Human HT-29 Cells
Immunocytochemical staining of intact living human colorectal adenocarcinoma (HT-29) cells. A. Extracellular staining of cells with Anti-Somatostatin Receptor Type 2 (extracellular) Antibody (#ASR-006), (1:50), (red). B. Merged image of A with live view of cell.
Expression of Orai1 in Rat Living RBL Cells
Immunocytochemical staining of intact living rat basophilic leukemia (RBL) cells. A. Extracellular staining of cells usingAnti-Orai1 (extracellular) Antibody (#ACC-062), (1:100) followed by goat anti-rabbit-AlexaFluor-488 secondary antibody (green). B. Merge of A and Live view of the cells.
Expression of GABA(A) α6 Receptor in Living Rat PC12 Cells
Immunocytochemical staining of intact living rat pheochromocytoma (PC12) cells. A. Extracellular staining of cells using Anti-GABA(A) α6 Receptor (extracellular) Antibody (#AGA-004), (1:100), (red). B. Merge of A with the live view of the cell.
Expression of P2X7 Receptor in Rat Living RBL Cells
Immunocytochemical staining of intact living rat basophilic leukemia (RBL) cells. A. Extracellular staining of cells using Anti-P2X7 Receptor (extracellular) Antibody (#APR-008), (1:100) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells.
Expression of Neurexin 1α in Rat Living PC12 Cells
Immunocytochemical staining of intact living rat pheochromocytoma (PC12) cells. A. Extracellular staining of cells using Anti-Neurexin 1α (extracellular) Antibody (#ANR-031), (1:50), (red). B. Merge of A with the live view of the cell.
Expression of CaV1.3 in Rat Living PC12 Cells
Immunocytochemical staining of intact living rat pheochromocytoma (PC12) cells. A. Extracellular staining of cells using Anti-CaV1.3 (CACNA1D) (extracellular) Antibody (#ACC-311), (1:50), (red). B. Merge of A with the live view of the cell.
Expression of TPC2 in Rat Living PC12 Cells
Immunocytochemical staining of intact living rat pheochromocytoma (PC12) cells. A. Extracellular staining of cells using Anti-TPCN2 Antibody (#ACC-072), (1:50), (red). B. Merge of A with the live view of the cell.
Expression of P2Y12 in Rat Living RBL Cells
Immunocytochemical staining of intact living rat basophilic leukemia (RBL) cells. A. Extracellular staining of cells using Anti-P2Y12 Receptor (extracellular) Antibody (#APR-020), (1:100) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells.
Expression of P2Y1 Receptor in Living Rat PC12 Cells
Immunocytochemical staining of intact living rat pheochromocytoma PC12 cells. A. Extracellular staining of cells using Anti-P2Y1 Receptor (extracellular) Antibody (#APR-021), (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Merge of extracellular staining with live view of the cells.
Expression of TRPC7 in Living Rat PC12 Cells
Immunocytochemical staining of intact living rat pheochromocytoma (PC12) cells using. A. Extracellular staining of cells using Anti-TRPC7 (extracellular) Antibody (#ACC-066), (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells.
Expression of TRPV2 in Living RBL Cells
Immunocytochemical staining of intact living rat basophilic leukemia (RBL) cells. A. Extracellular staining of cells using Guinea pig Anti-TRPV2 (VRL1) (extracellular) Antibody (#AGP-033), (1:100) followed by goat anti-guinea pig-AlexaFluor-488 secondary antibody (red). B. Live view of the cells. C. Merge image of A and B.
Expression of nAChRβ3 in Live Intact Rat PC12 Cells
Immunocytochemical staining of live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of cells with Anti-Nicotinic Acetylcholine Receptor β3 (CHRNB3) (extracellular) Antibody (#ANC-013), (1:50), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells. C. Merge image of A and B.
Expression of TRPA1 in Rat Living PC12 Cells
Immunocytochemical staining of intact living rat PC12 cells. A. Extracellular staining of cells using Anti-TRPA1 (extracellular) Antibody (#ACC-037), (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells. C. Merged image of A and B.
Expression of ZAC in Living Human PANC-1 Cells
Immunocytochemical staining of human intact living pancreatic adenocarcinoma (PANC-1) cells. Extracellular staining (green) of cells with Anti-Human Zinc-Activated Channel (ZAC) (extracellular) Antibody (#AZC-001), (1:25) followed by goat anti-rabbit-AlexaFluor-488 secondary antibody. DAPI is used as the counterstain (blue).
Expression of NaVβ4 in Living Rat PC12 Cells
Immunocytochemical staining of intact living rat pheochromocytoma (PC12) cells. A. Extracellular staining of cells using Anti-SCN4B (NaVβ4) (extracellular) Antibody (#ASC-044), (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Extracellular staining merged with live view of the cells.
Expression of P2X1 Receptor in Living Human SH-SYS5 Cells
Immunocytochemical staining of human intact living SH-SYS5 cells. A. Extracellular staining of cells with Anti-P2X1 Receptor (extracellular) Antibody (#APR-022), (1:50) followed by goat anti-rabbit-AlexaFluor-488 secondary antibody. B. Nuclear staining DAPI as the counterstain. C. Merged images of A and B.
Expression of GABA(A) α3 Receptor in Living Rat RIN-m Cells
Immunocytochemical staining of intact living rat RIN-m cells using Anti-GABA(A) α3 Receptor (extracellular) Antibody (#AGA-003), (1:50) followed by goat anti-rabbit-AlexaFluor-550 secondary antibody. Extracellular staining (red) merged with live view of the cells.
Expression of LH Receptor in Living Human OVCAR3 Cells
Immunocytochemical staining of intact living OVCAR3 cells. A. Extracellular staining of cells with Anti-LH Receptor (LHCGR) (extracellular) Antibody (#ALR-010), (1:25) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. B. Nuclear staining of cells using DAPI as the counterstain. C. Merged images of A and B.
Expression of CaVγ5 (CACNG5) in Living Rat PC12 Cells
Immunocytochemical staining of intact living rat PC12 cells using Anti-CACNG5 (extracellular) Antibody (#ACC-115), (1:25) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. A. Extracellular staining (red) merged with live view of the cells. B. Extracellular staining merged with nuclear staining using DAPI as the counterstain (blue).
Expression of Pannexin 2 in Living Human U-87 MG Glioblastoma Cell Line
Immunocytochemical staining of live intact human U-87 MG glioblastoma cells. A. Cells were stained with Anti-Pannexin 2 (extracellular) Antibody (#ACC-232), (1:50), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells. C. Merge of A and B.
Expression of mGluR1 in Living Rat C6 Glioma Cells
Immunocytochemical staining of live intact rat C6 glioma cells using Anti-mGluR1 (extracellular) Antibody (#AGC-006), (1:100), followed by goat-anti-rabbit-AlexaFluor-555 secondary antibody (red). Nuclei were stained with Hoechst 33342 (blue).
Expression of GABA(A) γ2 Receptor in Living Rat Hippocampal Neurons
TNF-α-induced downregulation of surface GABA(A) γ2 ReceptorImmunocytochemical staining of rat living hippocampal neurons. A. Extracellular staining of cells using Anti-GABA(A) γ2 Receptor (extracellular) Antibody (#AGA-005), (1:100). Representative images from time course experiments with cultured neurons undergoing TNF-α treatment ranging from 10 min to 6 h in duration. Results show a rapid downregulation in surface levels of the γ2 subunit in response to TNF-α. B. Quantification data of surface γ2 cluster area, over the 6 h time course of TNF-α treatment (n = 120 –158 images per time point; one-way ANOVA, Tukey’s post hoc test, **p < 0.01, ***p < 0.001).Adapted from Pribiag, H. and Stellwagen, D. (2013) with permission of the Society for Neuroscience.
Expression of NBC1 in Living Rat PC12 Cells
Immunocytochemical staining of live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of cells with Anti-SLC4A4 (NBC1) (extracellular) Antibody (#ANT-075), (1:100), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live image of the cells. C. Merge of the two images.
