Anti-CACNA1G (CaV3.1) Antibody

Voltage-dependent T-type calcium channel subunit α1G
Cat #: ACC-021
Alternative Name Voltage-dependent T-type calcium channel subunit α1G
  • KO Validated
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
      • Peptide MDEEEDGAGAEESGQPRSFTQL(C), corresponding to amino acid residues 1-22 of rat CACNA1G (Accession O54898). Intracellular, N-terminus.
    Accession (Uniprot) Number O54898
    Gene ID O54898
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical; human - 20/22 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, ih, wb
    May also work in: ifc, ip
      • Western blot analysis of rat brain membranes:
        1. Anti-CACNA1G (CaV3.1) Antibody (#ACC-021), (1:200).  
        2. Anti-CACNA1G (CaV3.1) Antibody, preincubated with the control peptide antigen.
      • Expression of CACNA1G in rat cerebellum
        Immunohistochemical staining of rat cerebellum using Anti-CACNA1G (CaV3.1) Antibody (#ACC-021). A. CACNA1G immunoreactivity (green) appears in the molecular layer. B. Nuclear staining using DAPI as the counterstain (blue). C. Merged images A and B. Mol = molecular layer.
        Human myometrium (1:200) (Blanks, A.M. et al. (2007) J. Physiol581, 915.).
      • Human myometrium cells (1:200) (Blanks, A.M. et al. (2007) J. Physiol581, 915.).
      • Voltage-dependent Ca2+ channels provide a pathway for rapid influx of Ca2+ into cells, which plays a crucial role in both electrical and metabolic signaling.1 

        T-type currents are transduced via channel proteins encoded by three genes that compose a subfamily within the CaV channel family.2-3 

        The activity of T-type channels contributes to several known physiological and pathophysiological phenomena including burst firing in neurons, pacemaking activity in the heart and secretion from endocrine tissues.2 There are three cloned T-type channel isoforms.  

        CACNA1G (CaV3.1) and CACNA1H (CaV3.2) are widely distributed whereas the expression of CACNA1I (CaV3.3) is restricted to the central nervous system.2

        CACNA1G and CACNA1H are also expressed in the kidney, but little is known about their physiological role there.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title: Anti-CACNA1G (Cav3.1) Antibody
    Expression of CaV3.1 in mouse pelvis kidney junction.
    Immunohistochemical staining of mouse kidney sections using Anti-CACNA1G (CaV3.1) Antibody (#ACC-021). A. CaV3.1 (purple) is detected in the pelvis kidney junction (PKJ). B. HCN3 staining (green), a marker of pacemaker cells, is detected in the PKJ. C. Same section stained for CaV3.1 (red). D. Merge of B and C panels shows high degree of co-localization between CaV3.1 and HCN3.
    Adapted from Hurtado, R. et al. (2014) with permission of Federation of American Societies for Experimental Biology.
    Last update: 08/07/2019

    Anti-CACNA1G (CaV3.1) Antibody (#ACC-021) is a highly specific antibody directed against an epitope of the rat CaV3.1 channel. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize CACNA1G from human, rat, and mouse samples.

    For research purposes only, not for human use
    Citations
      • Upregulation of CACNA1H under hypoxia.
        Immunocytochemical staining of rat pulmonary artery smooth muscle cells (PASMCs) and rat pheochromocytoma cells (PC12) using Anti-CaV3.2 (CACNA1H) Antibody (#ACC-025) and Anti-CACNA1G (CaV3.1) Antibody (#ACC-021). Staining shows upregulation of CACNA1H and not CACNA1G in response to oxygen stress.
        Adapted from Sellak, H. et al. (2014) with permission of the American Physiological Society.
      • Immunocytochemical staining of mouse endothelial cells. Tested in CaV3.1-/- mice.
        Gilbert, G. et al. (2017) Biochem. Pharmacol. 138, 61.
      • ND7/23 cell lysate (1:500).
        Mitani, K. et al. (2016) J. Pharmacol. Sci. 130, 177.
      • Mouse heart lysate.
        Walton, R.D. et al. (2016) J. Gerontol. A Biol. Sci. Med. Sci. 71, 1005.
      • Mouse brain lysates (1:1000).
        Rice, R.A. et al. (2014) Neurobiol. Aging 35, 1002.
      • Mouse HL-1 cells.
        Nguyen, N. et al. (2013) Biochim. Biophys. Acta 1833, 1294.
      • Rat retinal whole mounts (1:200).
        Fernandez, J.A. et al. (2015) Invest. Ophtalmol. Vis. Sci. 56, 5125.
      • Mouse kidney sections (1:700).
        Hurtado, R. et al. (2014) FASEB J. 28, 730.
      • Rat heart sections (1:20).
        Atkinson, A.J. et al. (2013) J. Am. Heart Assoc. 2, e000246.
      • Human myometrium (1:200).
        Blanks, A.M. et al. (2007) J. Physiol. 581, 915.
      • Mouse endothelial cells. Tested in CaV3.1-/- mice.
        Gilbert, G. et al. (2017) Biochem. Pharmacol. 138, 61.
      • Rat pulmonary artery smooth muscle cells (PASMCs) and rat pheochromocytoma cells (PC12), (1:100).
        Sellak, H. et al. (2014) Am. J. Physiol. 307, C648.
      • Human myometrium cells (1:200).
        Blanks, A.M. et al. (2007) J. Physiol. 581, 915.
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