Anti-DARPP32 Antibody

Dopamine and cAMP-regulated neuronal phosphoprotein, Protein phosphatase 1 regulatory subunit 1B, PPP1R1B
  • New
Cat #: ADR-006
Alternative Name Dopamine and cAMP-regulated neuronal phosphoprotein, Protein phosphatase 1 regulatory subunit 1B, PPP1R1B
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)EGPWERPPPLDESER, corresponding to amino acid residues 160 - 174 of human DARPP32 (Accession Q9UD71). Intracellular, C-terminus.
    • Anti-DARPP32 Antibody
    Accession (Uniprot) Number Q9UD71
    Gene ID 84152
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, rat – 13 out of 15 amino acid residues identical
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ih, wb
    May also work in: ic*, ip*
    Western blot
    • Anti-DARPP32 Antibody
      Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):
      1, 2. Anti-DARPP32 Antibody (#ADR-006), (1:200).
      3, 4. Anti-DARPP32 Antibody, preincubated with the negative control antigen.
    • Anti-DARPP32 Antibody
      Western blot analysis of human U-87 MG glioblastoma cell line lysate (lanes 1 and 3) and human HT-29 colorectal adenocarcinoma cell line lysate (lanes 2 and 4):
      1, 2. Anti-DARPP32 Antibody (#ADR-006), (1:200).
      3, 4. Anti-DARPP32 Antibody, preincubated with the negative control antigen.
    Immunohistochemistry
    • Anti-DARPP32 Antibody
      Expression of DARPP32 in rat striatum
      Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-DARPP32 Antibody (#ADR-006), (1:200), followed by goat anti-rabbit-AlexaFluor-488. DARPP32 immunoreactivity (green) appears in striatal cells (arrows). ST=striatum, CC=corpus callosum. Cell nuclei are stained with DAPI (blue).
    References
    1. Avanes, A. et al. (2019) Biochem. Pharmacol. 160, 71.
    2. Yamada, K. et al. (2016) J. Physiol. Sci. 66, 435.
    3. Momand, J. et al. (2017) FEBS Open Bio 7, 1328.
    4. Greengard, P. et al. (1999) Neuron 23, 435.
    5. Belkhiri, A. et al. (2016) Oncotarget 7, 17631.
    6. Svenningsson, P. et al. (2004) Annu. Rev. Pharmacol. Toxicol. 44, 269.
    Scientific background

    Dopamine- and cAMP-regulated neuronal phosphoprotein (DARPP32), also known as, Protein phosphatase 1 regulatory subunit 1B (PPP1R1B) is a protein regulated by the phosphorylation of cAMP1.

    DARPP32 is expressed in the spiny neurons of the neostriatum and in the adrenal medulla, kidney, and parathyroid cells and it is also overexpressed in tumor cells from breast, colon, esophagus, lung and prostate tissues1,2. Its protein structure is comprised of 18% alpha helix, 23% beta strand, 30% beta turn, and 29% random coil3.

    DARPP32 has been shown to play a key role in mediating the biochemical, electrophysiological, and behavioral effects of dopamine on dopaminoceptive neurons4,5. It is also involved in mediating the actions of other neurotransmitter systems such as serotonin and glutamate, in response to a variety of drugs5,6.

    DARPP32 is reported to have a role in development and neurodegenerative diseases. In addition, DARPP32 plays a role in cancer tumorigenesis. Collectively, findings from published reports suggest DARPP32 as a potential biomarker of drug resistance and a therapeutic target in gastric cancer and other malignancies5.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat

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    Last update: 11/02/2020

    Anti-DARPP32 Antibody (#ADR-006) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize DARPP32 from human, rat, and mouse samples.

    For research purposes only, not for human use
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