Anti-Human TRPV6 (extracellular) Antibody

Epithelial calcium channel 2, ECaC2, Calcium transport protein 1, CaT1, CaT-like, CaT-L, Transient receptor potential cation channel subfamily V member 6
    Cat #: ACC-028
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h
    Immunogen
    Peptide (C)RTNNRTSPRDN, corresponding to amino acid residues 395-405 of human TRPV6 (Accession Q9H1D0). 1st extracellular loop.
    Accession (Uniprot) Number Q9H1D0
    Gene ID 55503
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Specificity Not recommended for use in mouse and rat samples.
    Slight possibility, if any, for some cross reactivity with human TRPV5.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.85 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, lci, wb
    May also work in: ifc, ih, ip
    Western blot
    Western blot analysis of human prostate carcinoma cell lines (LNCaP, DU145, PC-3) lysates:
    1-3. Anti-Human TRPV6 (extracellular) Antibody (#ACC-028), (1:200).
    4-6. Anti-Human TRPV6 (extracellular) Antibody, preincubated with the control peptide antigen.
    Live cell imaging / Immunocytochemistry
    Expression of TRPV6 in human LNCaP cells
    Immunocytochemical staining of intact living human prostate carcinoma (LNCaP) cells. A. Extracellular staining of cells using Anti-Human TRPV6 (extracellular) Antibody (#ACC-028), (1:50), followed by goat anti-rabbit-AlexaFluor-488 secondary antibody (green). B. Merge images of A with live view of the cells.
    References
    1. Montell, C. et al. (2002) Mol. Cell. 9, 229.
    2. Clapham, D.E. (2003) Nature 426, 517.
    3. Moran, M.M. et al. (2004) Curr. Opin. Neurobiol14, 362.
    4. Clapham, D.E. et al. (2003) Pharmacol. Rev55, 591.
    5. Gunthorpe, M.J. et al. (2002) Trends. Pharmacol. Sci23, 183.
    6. Hoenderop, J.G. et al. (2003) Pflugers. Arch. 446, 304.
    7. Peng, J.B. et al. (2003) J. Physiol. 551, 729.
    8. Fixemer, T. et al. (2003) Oncogene 22, 7858.
    9. Wissenbach, U. et al. (2001) J. Biol. Chem276, 19461.
    Scientific background

    TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1 According to IUPHAR the TRP family comprises of three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family; the TRPA, TRPML, and TRPP).1-4 The TRPV subfamily consists of six members, TRPV1-6.5

    TRPV6 (CaT1) and TRPV5 (ECaC) are unique members of the TRP family. Both are epithelial calcium channels, form constitutively open channels, share high degree of homology (about 66%) with differences in the N and C-terminus. TRPV6 and TRPV5 are expressed in a 1,25-dihydroxyvitamin D3 dependent manner. In contrast to other members of the TRP family, TRPV6 and TRPV5 are highly Ca2+ selective and are expressed in Ca2+ transporting epithelia where they are assumed to have an important role in Ca2+ reabsorption. TRPV6 is highly expressed in the placenta, small intestine and kidney.6,7

    While not being expressed in benign prostate tissue, TRPV6 was found to be upregulated in prostate cancer tissue and correlation between expression and tumor grade was shown. Finally, TRPV6 was suggested as prognostic marker and as a promising candidate for therapeutic strategies.8,9

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 10/12/2018

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the human TRPV6 channel. Anti-Human TRPV6 (extracellular) Antibody (#ACC-028) can be used in western blot and live cell imaging applications. It has been designed to recognize TRPV6 from human samples. The antibody is unlikely to recognize the channel from rat and mouse samples. In addition, there is a slight possibility, if any, for some cross-reactivity with human TRPV5.

    For research purposes only, not for human use
    Citations
    Related Products
      • Products for control experiments
        1. Anti-TRPV6 Antibody (#ACC-036). This antibody recognizes a different epitope and thus can also be used as a control for detecting TRPV6.