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Anti-KCNH7 (erg3) Antibody

KV11.3, Potassium voltage-gated channel subfamily H member 7, Ether-a-go-go-related channel 3, Eag-related protein 3, ERG-3, hERG3
Cat #: APC-112
Alternative Name KV11.3, Potassium voltage-gated channel subfamily H member 7, Ether-a-go-go-related channel 3, Eag-related protein 3, ERG-3, hERG3
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide CPEFLDLEKSKLKSKE, corresponding to amino acid residues 1108-1123 of rat KV11.3 (erg3) (Accession O54852). Intracellular, C-terminal part.
Accession (Uniprot) Number O54852
Gene ID 170739
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Human - identical; mouse - 15/16 amino acid residues identical.
RRID AB_2039937.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.6 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ih, wb
May also work in: ifc*, ip*
Western blot
  • Western blot analysis of rat brain lysate:
    Western blot analysis of rat brain lysate:
    1. Anti-KCNH7 (erg3) Antibody (#APC-112), (1:300).
    2. Anti-KCNH7 (erg3) Antibody, preincubated with KCNH7/erg3 Blocking Peptide (#BLP-PC112).
Immunohistochemistry
  • Expression of KV11.3 (erg3) in rat cerebellum
    Expression of KV11.3 (erg3) in rat cerebellum
    Immunohistochemical staining of rat cerebellum using Anti-KCNH7 (erg3) Antibody (#APC-112). A. KV11.3 channel appears in glial processes (red). B. Staining of astrocytic fibers with mouse anti-glial fibrillary acidic protein (GFAP, green). C. Confocal merge of KV11.3 channel and GFAP demonstrates colocalization in the molecular layer but separate localization of these proteins at the Purkinje cell layer.
  • Mouse vomeronasal organ (VNO) (1:250) (Hagendorf, S. et al. (2009) J. Neurosci. 29, 206.).
Immunocytochemistry
  • Rat INS-1 cells (1:50) (Muhlbauer, E. et al. (2007) Cell. Mol. Life Sci64, 768.).
References
  1. Shi. W.M. et al. (1997) J. Neurosci. 17, 9423.
  2. Wimmers, S. et al. (2001) Pflugers Arch. 441, 450.
  3. Papa, M. et al. (2003) J. Comp. Neurol. 466, 119.
Scientific background

KV11.3 (erg3) is a member of the ether-a-go-go (EAG) subfamily of voltage-dependent K+ channels. The erg subfamily includes the closely related proteins KV11.1 (erg1) and KV11.2 (erg2) that possess the signature structure of the voltage-dependent K+ channels: six membrane-spanning domains with intracellular N- and C-termini. As with all voltage-dependent K+ channels, the functional channel is a tetramer composed of four subunits. It has been suggested that the KV11 subfamily members can form functional heteromultimers within the subfamily.

The current of the KV11.1 and KV11.2 channels is that of a strong inward rectifier with slow activating kinetics. The KV11.3 channel has different biophysical properties: it is a weak inward rectifier that is activated at negative potentials and has rapid activating kinetics.

KV11.3 expression is believed to be concentrated mainly in the brain with the stronger expression detected in the cerebral cortex and hippocampus.

Currently, there is not much pharmacological information concerning KV11.3 modulation, but the channel has been shown to be inhibited (as are all KV11 subfamily members) by the anti-arrhythmic E-4031 as well as by the anti-psychotic drugs sertindole and pimozide.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 16/08/2020

Alomone Labs is pleased to offer Anti-KCNH7 (erg3) Antibody (#APC-112) that is directed against a highly conserved epitope of the rat KV11.3 channel. The antibody will also react with human and mouse samples. The antibody can be used in western blot, immunohistochemistry and immunocytochemistry applications. 

For research purposes only, not for human use
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