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Anti-SLC25A12 Antibody

AGC1, ARALAR1, Solute carrier family 25 member 12, Mitochondrial aspartate glutamate carrier 1, Calcium-binding mitochondrial carrier protein Aralar1, Araceli hiperlarga

Cat #: ANT-112
Alternative Name AGC1, ARALAR1, Solute carrier family 25 member 12, Mitochondrial aspartate glutamate carrier 1, Calcium-binding mitochondrial carrier protein Aralar1, Araceli hiperlarga
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)KDKSKSGMISGLD, corresponding to amino acid residues 169-181 of mouse SLC25A12 (Accession Q8BH59). Intracellular, N-terminus.
Accession (Uniprot) Number Q8BH59
Gene ID 78830
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, human – identical.
RRID AB_2756727.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Western blot analysis of rat skeletal muscle lysate (lanes 1 and 4, 1:600), mouse brain membrane (lanes 2 and 5) and human OVCAR3 ovarian adenocarcinoma cell line lysate (lanes 3 and 6):
    Western blot analysis of rat skeletal muscle lysate (lanes 1 and 4, 1:600), mouse brain membrane (lanes 2 and 5) and human OVCAR3 ovarian adenocarcinoma cell line lysate (lanes 3 and 6):
    1-3. Anti-SLC25A12 Antibody (#ANT-112), (1:200).
    4-6. Anti-SLC25A12 Antibody, preincubated with SLC25A12 Blocking Peptide (#BLP-NT112).
Immunohistochemistry
  • Expression of SLC25A12 in rat hippocampus.
    Expression of SLC25A12 in rat hippocampus.
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-SLC25A12 Antibody(#ANT-112), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the rat hippocampal dentate gyrus region, showed immunoreactivity (green) in neurons of the hilus (H, arrows). B. Pre-incubation of the antibody with SLC25A12 Blocking Peptide (#BLP-NT112), suppressed staining. Cell nuclei are stained with DAPI (blue). 
    G = granule layer.
     
References
  1. Palmieri, F. (2013) Mol. Aspects Med. 34, 465.
  2. Napolioni, V. et al. (2011) Mol. Neurobiol. 44, 83.
Scientific background

SLC25 is a large family of nuclear-encoded transporters embedded in the inner mitochondrial membrane and in a few cases other organelle membranes.

SLC2512A is a gene encoding the aspartate/glutamate carrier 1 (AGC1). AGC1 possesses six transmembrane alpha helices and N- and C-termini on the cytosolic side of the membrane1.

AGC1 catalyzes an exchange between intra-mitochondrial aspartate and cytosolic glutamate, an important step in urea synthesis. As a component of the malate–aspartate shuttle (MAS), AGC1 also has a role in the transfer of NADH, carrying reducing equivalents from the cytosol into the mitochondrial matrix. AGC1 is expressed predominantly in heart and skeletal muscle, with weaker expression in brain and kidney. AGC1 is the main AGC isoform present in the adult brain, and it is expressed mainly in neurons. As part of the MAS, AGC1 has a role in the physiology of pancreatic β-cells by influencing glucose-induced activation of mitochondrial metabolism and insulin secretion and it is the only AGC isoform expressed in pancreatic islets and β-cells. 

AGC1 has several Ca2+-binding EF-hand motifs located on its long, hydrophilic amino-terminus which extends into the intermembrane space and its activity is strongly regulated by extra-mitochondrial Ca2+. Dysregulation of cellular Ca2+ homeostasis mediated by AGC1 has been implicated in the pathogenesis of autistic spectrum disorder2.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/04/2021

Anti-SLC25A12 Antibody (#ANT-112) is a highly selective antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize AGC1 from human, mouse, and rat samples.

For research purposes only, not for human use

Applications

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Scientific Background

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