Anti-sloβ4 (KCNMB4) Antibody

Ca2+-activated K+ channel subunit β4, BKCa β subunit 4, BK beta4, Maxi K channel β4, Charybdotoxin receptor subunit beta-4
Cat #: APC-061
Alternative Name Ca2+-activated K+ channel subunit β4, BKCa β subunit 4, BK beta4, Maxi K channel β4, Charybdotoxin receptor subunit beta-4
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
      • Peptide AKLRVSYEYTEAEDKS(C), corresponding to amino acid residues 2-17 of rat KCNMB4 (Accession Q9ESK8). Intracellular, N-terminal part.
    Accession (Uniprot) Number Q9ESK8
    Gene ID 66016
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human - identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, ih, wb
    May also work in: ifc, ip
      • Western blot analysis of rat brain membranes:
        1. Anti-sloβ4 (KCNMB4) Antibody (#APC-061), (1:200).
        2. Anti- sloβ4 (KCNMB4) Antibody, preincubated with the control peptide antigen.
        Human detrusor smooth muscle (DSM) cells (1:200) (Hristov, K.L. et al. (2011) Am. J. Physiol. 301, C903.).
      • Expression of KCNMB4 in rat hippocampus and cortex

        Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-sloβ4 (KCNMB4) Antibody (#APC-061), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. A. KCNMB4 staining (green) in rat hippocampal CA1 region is detected in apical dendrites (arrows) of pyramidal neurons from the pyramidal layer (PL). B. Staining in rat parietal cortex shows staining of apical dendrites (arrows) of pyramidal neurons. Cell nuclei are stained with DAPI (blue).

        Rat brain sections (1:50) (Piwonska, M. et al. (2008) Neuroscience 153, 446.).
      • Human detrusor smooth muscle (DSM) cells (1:100) (Hristov, K.L. et al. (2011) Am. J. Physiol. 301, C903.).
      • Meera, P. et al. (2000) Proc. Natl. Acad. Sci. U.S.A. 97, 5562.
      • Orio, P. et al. (2002) News Physiol. Sci. 17, 156.
      • Brenner, R. et al. (2000) J. Biol. Chem. 275, 6453.
      • sloβ4 is a member of a family of regulatory β subunits that control the activity of the large conductance Ca2+-activated K+ channel, KCa1.1. The family includes four members with a shared topology: two transmembrane domains, short intracellular N- and C-termini and a large extracellular region.

        The four members of the family have a distinct tissue distribution with sloβ4 expressed almost exclusively in the central nervous system (CNS).

        Functionally, sloβ4 increases the sensitivity of the pore-forming KCa1.1 subunit to Ca2+ and voltage and it also changes its pharmacology. It has been shown that co-expression of sloβ4 with KCa1.1 makes the latter resistant to nanomolar concentrations of the well-known inhibitors charybdotoxin and iberiotoxin.

        The physiological significance of sloβ4 expression in the CNS is not clear, but KCa1.1 channels are likely involved in the regulation of neurotransmitter release in presynaptic terminals.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 10/06/2019

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat sloβ4. Anti-sloβ4 (KCNMB4) Antibody (#APC-061) can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It was designed to recognize sloβ4 from human, rat, and mouse samples.

    For research purposes only, not for human use
    Citations
      • Human dermal fibroblasts (1:200).
        Kicinska, A. et al. (2016) Biochem. J. 473, 4457.
      • Mouse olfactory bulb lysate.
        Gorin, M. et al. (2016) J. Neurosci. 36, 3127.
      • Human U-87 MG cell lysate (1:200).
        Bednarczyk, P. et al. (2013) PLoS ONE 8, e68125.
      • Human detrusor smooth muscle (DSM) cells (1:200).
        Hristov, K.L. et al. (2011) Am. J. Physiol. 301, C903.
      • Mouse olfactory bulb sections (1:100).
        Gorin, M. et al. (2016) J. Neurosci. 36, 3127.
      • Rat brain sections (1:50).
        Piwonska, M. et al. (2008) Neuroscience 153, 446.
      • Rat pinealocytes.
        Mizutani, H. et al. (2016) Am. J. Physiol. 310, C740.
      • Human detrusor smooth muscle (DSM) cells (1:100).
        Hristov, K.L. et al. (2011) Am. J. Physiol. 301, C903.
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