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Anti-Syntaxin 3 Antibody

STX3

Cat #: ANR-005
Alternative Name STX3
  • KO Validated
  • Lyophilized Powder yes
    Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide KDRLEQLKAKQLTQD(C), corresponding to amino acid residues 2-16 of rat syntaxin 3 (Accession Q08849). Cytoplasmic, N-terminus.
    Accession (Uniprot) Number Q08849
    Gene ID 81802
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human - identical.
    RRID AB_2040228.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.3 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, wb
    May also work in: ifc*, ih*, ip*
    Western blot
    • Western blot analysis of rat brain (lanes 1 and 3) and kidney (lanes 2 and 4) membranes:
      Western blot analysis of rat brain (lanes 1 and 3) and kidney (lanes 2 and 4) membranes:
      1,2. Anti-Syntaxin 3 Antibody (#ANR-005), (1:200).
      3,4. Anti-Syntaxin 3 Antibody, preincubated with Syntaxin 3 Blocking Peptide (#BLP-NR005).
    Immunocytochemistry
    • Human MDA-MB-231 cells (Williams, K.C. et al. (2014) Mol. Biol. Cell 25, 2061.).
    References
    1. Jahn, R. and Scheller, R.H. (2006) Nat. Rev. Mol. Cell Biol. 7, 631.
    2. Südhof, T.C. and Rothman, J.E. (2009) Science 323, 474.
    3. Weisz, O.A. and Rodriguez-Boulan, E. (2009) J. Cell. Sci. 122, 4253.
    Scientific background

    Syntaxin 3 is a member of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) protein superfamily. The family includes 36 members in humans and is characterized by the SNARE motif, an evolutionarily conserved stretch of 60–70 amino acids that are arranged in heptad repeats1,2. SNARE proteins are involved in exocytosis and intracellular vesicle trafficking and are essential for cell growth, hormone secretion and neurotransmission, processes that require rapid, targeted, and regulated membrane fusion1,2.

    SNAREs can be roughly divided into vesicular (v-SNAREs) and target (t-SNAREs) based on their distribution on the transport vesicle or target membrane respectively. Thus, assembly of cognate v-/t-SNAREs between two opposing membranes generates trans-SNARE complexes, which bring the lipid bilayers in close proximity and drive membrane fusion.

    Syntaxin 3, like most SNAREs, is a type IV membrane protein with a relatively large N-terminus containing the SNARE motif located in the cytoplasmic side and a transmembrane domain located close to the C-terminus that functions as an anchor1,2.

    Syntaxin 3 functions as a plasma membrane protein t-SNARE that is involved in epithelial cell polarization. Indeed, Syntaxin 3 is specifically localized in the apical membrane of polarized epithelial cells where it likely has a role in the polarized trafficking of newly synthesized proteins3.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 08/01/2023

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Syntaxin 3. Anti-Syntaxin 3 Antibody (#ANR-005) can be used in western blot and immunocytochemistry applications. It has been designed to recognize Syntaxin 3 from rat, mouse and human samples.

    For research purposes only, not for human use

    Applications

    Citations

    Citations
    KO validation citations
    1. Western blot of human AD293 and Caco-2 cells. Tested in cells transfected with Syntaxin 3 (STX3) siRNA. 
      MotoikeS. et al. (2021J. Pharmacol. Sci. 145, 297. 
    Immunocytochemistry citations
    1. Human MDA-MB-231 cells.
      Williams, K.C. et al. (2014) Mol. Biol. Cell 25, 2061.

    Specifications

    Scientific Background

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