Cat #: BLP-SC022
Type: Synthetic peptide
Form: Lyophilized powder
A Blocking peptide for Anti-NALCN/VGCNL1 (extracellular) Antibody (#ASC-022). Intended to be used as a negative control.
For research purposes only, not for human use
- Western blot analysis of rat brain (lanes 1 and 4), mouse heart (lanes 2 and 5) and SH-SYS5 cell (lanes 3 and 6) lysates:1-3. Anti-NALCN/VGCNL1 (extracellular) Antibody (#ASC-022), (1:200).
4-6. Anti-NALCN/VGCNL1 (extracellular) Antibody, preincubated with NALCN/VGCNL1 (extracellular) Blocking Peptide (#BLP-SC022).
- Expression of NALCN in rat hypothalamusImmunohistochemical staining of rat hypothalamus using Anti-NALCN/VGCNL1 (extracellular) Antibody (#ASC-022). A. NALCN (green) staining appears in the supra-optic nucleus (SON). B. DAPI is used as the counterstain (blue). White arrows point at the sharp border between the SON and optic tract (OT). C. Merged images of A and B.
- (C)SMMFESPFRRVMH, corresponding to amino acid residues 902-914 of rat NALCN (Accession Q6Q760).
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
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