NALCN/VGCNL1 (extracellular) Blocking Peptide (#BLP-SC022) is the original antigen used for immunization during Anti-NALCN/VGCNL1 (extracellular) Antibody (#ASC-022) generation. The blocking peptide binds and ‘blocks’ Anti-NALCN/VGCNL1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain (lanes 1 and 4), mouse heart (lanes 2 and 5) and human SH-SYS5 brain neuroblastoma cell (lanes 3 and 6) lysates:
- Expression of NALCN in rat hypothalamusImmunohistochemical staining of rat hypothalamus using Anti-NALCN/VGCNL1 (extracellular) Antibody (#ASC-022). A. NALCN (green) staining appears in the supra-optic nucleus (SON). B. DAPI is used as the counterstain (blue). White arrows point at the sharp border between the SON and optic tract (OT). C. Merged images of A and B.
- (C)SMMFESPFRRVMH, corresponding to amino acid residues 902-914 of rat NALCN (Accession Q6Q760).