Overview
Cat #: BLP-NR062
Type: Synthetic peptide
Form: Lyophilized powder
Neuropilin-2/NRP2 (extracellular) Blocking Peptide (#BLP-NR062) is the original antigen used for immunization during Anti-Neuropilin-2 (NRP2) (extracellular) Antibody (#ANR-062) generation. The blocking peptide binds and ‘blocks’ Anti-Neuropilin-2/NRP2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain synaptosomal fraction (lanes 2 and 4):1,2. Anti-Neuropilin-2 (NRP2) (extracellular) Antibody (#ANR-062), (1:200).
3,4. Anti-Neuropilin-2 (NRP2) (extracellular) Antibody, preincubated with Neuropilin-2/NRP2 (extracellular) Blocking Peptide (#BLP-NR062). - Western blot analysis of human U-87 MG glioma cell line lysate:1. Anti-Neuropilin-2 (NRP2) (extracellular) Antibody (#ANR-062), (1:200).
2. Anti-Neuropilin-2 (NRP2) (extracellular) Antibody, preincubated with Neuropilin-2/NRP2 (extracellular) Blocking Peptide (#BLP-NR062). - Expression of Neuropilin-2 in mouse substantia nigra pars compactaImmunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-Neuropilin-2 (NRP2) (extracellular) Antibody (#ANR-062), (1:400). A. NRP2 staining (green) reveals several neuronal profiles of NRP2 positive neurons that are distributed along the substantia nigra pars compacta. B. The same section stained for Calbindin-D28K (red) shows positive neurons along the substantia nigra pars compacta. C. Merge of the two images reveals co-localization in several neurons (arrows). DAPI counterstain is used to visualize nuclei of all cells (blue).
Properties
Sequence
Accession (Uniprot) Number O60462
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.