Ion Channels

Ion Channels

Alomone Labs offers one of the most comprehensive, in-house-made portfolios of primary antibodies in the ion channel field, generated in rabbit, mouse, and guinea pig hosts covering voltage-gated and ligand-gated ion channels, regulatory subunits, and related proteins. All antibodies are produced and rigorously validated internally, ensuring high specificity and consistent performance across applications such as Western blotting, immunohistochemistry, immunocytochemistry, flow cytometry, and more. To further support advanced imaging and multiplexing, we also provide a growing selection of fluorophore-conjugated antibody options. This extensive suite of in-house products empowers researchers to precisely detect, visualize, and characterize ion channel expression and function in diverse biological systems.

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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Category: Connexin Family
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22 Results
  • Anti-Connexin-26 (GJB2) Antibody
    Anti-Connexin-26 (GJB2) Antibody
    A Rabbit Polyclonal Antibody to Connexin-26
    • Product Code: ACC-212
    • Short Description: A Rabbit Polyclonal Antibody to Connexin-26
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: CX26, Gap junction β2 protein
  • Anti-Connexin-29 (extracellular) Antibody
    Anti-Connexin-29 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Connexin-29
    • Product Code: ACC-223
    • Short Description: A Rabbit Polyclonal Antibody to Connexin-29
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: CX29, Gap junction γ3 protein, Gap junction ε1 protein, GJC3, GJE1
  • Anti-Connexin-31 (GJB3) Antibody
    Anti-Connexin-31 (GJB3) Antibody
    A Rabbit Polyclonal Antibody to Connexin-31
    • Product Code: ACC-213
    • Short Description: A Rabbit Polyclonal Antibody to Connexin-31
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: CX31, Gap junction β3 protein
  • Anti-Connexin-32 Antibody
    Anti-Connexin-32 Antibody
    A Rabbit Polyclonal Antibody to Connexin-32
    • Product Code: ACC-211
    • Short Description: A Rabbit Polyclonal Antibody to Connexin-32
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: CX32, Gap junction β1 protein, GJB1, CMTX
  • Anti-Connexin-36 Antibody
    Anti-Connexin-36 Antibody
    A Rabbit Polyclonal Antibody to Connexin-36
    • Product Code: ACC-209
    • Short Description: A Rabbit Polyclonal Antibody to Connexin-36
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: CX36, Gap junction δ2 protein, Gap junction α9 protein, GJD2, GJA9
  • Anti-Connexin-36-ATTO Fluor-488 Antibody
    Anti-Connexin-36-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to Connexin-36 Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: ACC-209-AG
    • Short Description: A Rabbit Polyclonal Antibody to Connexin-36 Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: CX36, Gap junction δ2 protein, Gap junction α9 protein, GJD2, GJA9
  • Anti-Connexin-37 Antibody
    Anti-Connexin-37 Antibody
    A Rabbit Polyclonal Antibody to Connexin-37
    • Product Code: ACC-204
    • Short Description: A Rabbit Polyclonal Antibody to Connexin-37
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: CX37, Gap junction α4 protein, GJA4
  • Anti-Connexin-40 (GJA5) Antibody
    Anti-Connexin-40 (GJA5) Antibody
    A Rabbit Polyclonal Antibody to Connexin-40
    • Product Code: ACC-205
    • Short Description: A Rabbit Polyclonal Antibody to Connexin-40
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: CX40, Gap junction α5 protein
  • Anti-Connexin-43 Antibody
    Anti-Connexin-43 Antibody
    A Rabbit Polyclonal Antibody to Connexin-43
    • Product Code: ACC-201
    • Short Description: A Rabbit Polyclonal Antibody to Connexin-43
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: CX43, Gap junction α1 protein, GJA1, GJAL, Gap junction 43 kDa heart protein
  • Anti-Connexin-45 (GJC1) Antibody
    Anti-Connexin-45 (GJC1) Antibody
    A Rabbit Polyclonal Antibody to Connexin-45
    • Product Code: ACC-207
    • Short Description: A Rabbit Polyclonal Antibody to Connexin-45
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: CX45, Gap junction γ1 protein, Gap junction α7 protein, GJA7
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