Biotin Custom Conjugation
Sometimes what you need isn’t in the catalog. Our custom services – including antibody and toxin conjugation, bulk ordering, and lot reservation –deliver the exact format, label, and quantity you need, without changing suppliers or validation standards.
Biotin Custom Conjugation
Primary biotin-conjugated antibodies produce an amplified immunodetection by binding streptavidin molecules and can be used for many applications. Amplified signals allow detection of proteins expressed at low levels, increasing the sensitivity of immunodetection tests.
Customizable
Custom antibody and toxin conjugation to fluorophores, enzymes, or biotin, tailored to your specific assay and experimental design.
Conjugation Ready
Well-characterized antibodies and toxins optimized for conjugation while preserving binding specificity, biological activity, and detection performance.
Broadly Compatibility
Suitable for immunofluorescence, flow cytometry, immunoassays, and multiplex detection workflows using directly labeled reagents.
Consistent Quality
Controlled production, bulk ordering, and lot reservation options ensure long-term consistency and reproducibility across experiments.
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Uses & Applications
Our custom conjugation services support antibody- and toxin-based detection across a wide range of research applications, enabling direct, sensitive, and reproducible results. These conjugated reagents are optimized for direct detection and multiplex assay design.
Immunofluorescence (IF, ICC, IHC)
Conjugated reagents enable low-background immunofluorescence and immunohistochemistry without secondary antibodies.
Western Blot & Blot-Based Assays
Biotin-conjugated primary antibodies enable amplified detection in western blot and blot-based assays.
ELISA
Biotin-conjugated antibodies support sensitive and reproducible ELISA-based detection.
Multiplex Immunodetection
Simultaneous multi-target detection using spectrally distinct fluorophore, enzyme, or biotin conjugation for multiplex assays.
Flow Cytometry & Imaging Flow Cytometry
Custom-labeled antibodies support flow cytometry panel design and robust multiplex analysis of surface and intracellular markers.
Pull-Down & Capture Assays
Biotin-conjugated antibodies and toxins support streptavidin-based pull-down and protein interaction studies.
In Vivo
Custom conjugation allows tailored labeling for selected in vivo studies, improving consistency and signal quality.
Tissue Clearing
Optimized labeling strategies for tissue clearing, improving signal penetration and image clarity.
Biotin Custom Conjugation
This service is suitable for colorimetric, chemiluminescent, and pull-down applications
Service Notes
Minimum order: 4 × 50 µl.
Rabbit IgG isotype controls can be biotin-labeled for your application.
| Conjugate Type | Excitation (nm) | Emission (nm) | Emission Color | Recommended Lasers (nm) |
| Fluorescent Conjugates | ||||
| mFluor Violet™ 450 | 407 | 455 |
Violet / Blue
|
405 |
| FITC | 494 | 518 |
Green
|
488 |
| ATTO Fluor-488 | 500 | 520 |
Green
|
561 |
| ATTO Fluor-550 | 554 | 578 |
Yellow-Orange
|
561 |
| R-PE | 561 | 578 |
Orange
|
561 |
| ATTO Fluor-590 | 593 | 622 |
Orange-Red
|
561 |
| ATTO Fluor-594 | 603 | 626 |
Orange-Red
|
561 |
| ATTO Fluor-633 | 630 | 651 |
Red
|
640 |
| APC | 633-640 | 660 |
Red
|
640 |
| ATTO Fluor-647N | 646 | 664 |
Far-Red
|
640 |
| iFluor® 800 | 801 | 820 |
Near-Infrared
|
785/808 |
| Non-Fluorescent Conjugates | ||||
| Biotin | - | - | - | - |
| HRP | - | - | - | - |
| Alkaline Phosphatase | - | - | - | - |
Data Highlights
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Get Your Custom Quote
Validated conjugation to fluorophores, enzymes, or biotin for antibodies and toxins, with flexible quantities,
bulk pricing, and lot reservation tailored to your needs.
FAQs
View all FAQs >
If you can’t find the answer, please contact us.
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Quality control is routinely performed on the unconjugated antibody using Western blot (WB) to verify specificity and performance. If validation of the conjugated product is required, it can be performed upon request for an additional fee.
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Yes. The Degree of Labeling (DOL) value can be provided when this information is available for the specific conjugated product.
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To assist with experimental design and fluorophore selection, you can use the following tools:
- Panel Builder – to help design optimized multi-color panels.
- Spectra Viewer – to visualize fluorophore excitation and emission spectra.
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If you require a custom conjugation that is not listed, please contact us with your request at [email protected]. Our team will be happy to evaluate your needs and discuss possible solutions.