Extracellular antibodies are ideal research tools for cell-surface proteins.
No permeabilization/fixation because using extracellular antibodies allows you to work with live cells!
Benefits of live cell imaging:
✓ Monitor cell movement
✓ Study protein transport across the cell
✓ Investigate protein internalization
L-type Ca2+ channels include four different isoforms of the α1 pore-forming subunit: CaV1.1, CaV1.2, CaV1.3 and CaV1.4. Their expression is detected in muscle, heart, retina and neurons where they play important roles in muscle contraction, hormone secretion, neuronal excitability and gene expression.