Cell Surface-Binding Antibodies

Cell Surface-Binding Antibodies

Antibodies targeting extracellular epitopes for surface labeling and live-cell detection

Cell surface-binding antibodies are essential tools for studying membrane proteins in their native, physiological context. These in-house produced antibodies recognize extracellular epitopes of cell surface proteins, enabling live-cell labeling, flow cytometry, surface receptor tracking, and functional studies without the need for cell permeabilization.
Alomone Labs offers a comprehensive portfolio of highly specific, well-validated antibodies targeting extracellular domains of ion channels, receptors, and transporters. These antibodies are carefully designed to ensure epitope accessibility, specificity, and reproducibility across advanced imaging and cytometry workflows. Each antibody is rigorously validated in immunohistochemistry (IHC), immunocytochemistry (ICC), western blot (WB), and/or flow cytometry, with matched blocking peptides available to confirm specificity.

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FAQs

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.
  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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803 Results
  • Anti-SLC35G1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to SLC35G1 Transporter
    • Product Code: ANT-196
    • Short Description: A Rabbit Polyclonal Antibody to SLC35G1 Transporter
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Solute carrier family 35 member G1, Partner of STIM1, POST, Transmembrane protein 20, C10orf60, TMEM20
  • Anti-LAT3/SLC43A1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to LAT3 Transporter
    • Product Code: ANT-193
    • Short Description: A Rabbit Polyclonal Antibody to LAT3 Transporter
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Large neutral amino acids transporter small subunit 3, L-type amino acid transporter 3, Solute carrier family 43 member 1
  • Anti-SLC3A2 (CD98) (extracellular) Antibody
    A Rabbit Polyclonal antibody to CALHM2 (extracellular)
    • Product Code: ANT-192
    • Short Description: A Rabbit Polyclonal antibody to CALHM2 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Solute Carrier Family 3 Member 2, 4F2 Cell-Surface Antigen Heavy Chain, 4F2HC, CD98 Antigen Heavy Chain, CD98HC
  • Anti-CAT-1 (SLC7A1) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to CAT-1 (SLC7A1) (extracellular)
    • Product Code: ANT-191
    • Short Description: A Rabbit Polyclonal Antibody to CAT-1 (SLC7A1) (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot, Immunohistochemistry
    • Target: SLC7A1
  • Anti-SLC10A4 (extracellular) Antibody
    A Rabbit Polyclonal antibody to SLC10A4 (extracellular)
    • Product Code: ANT-174
    • Short Description: A Rabbit Polyclonal antibody to SLC10A4 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Sodium/Bile Acid Cotransporter 4, P4, Solute Carrier Family 10 Member 4
  • Anti-SLC41A2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to SLC41A2 Transporter
    • Product Code: ANT-146
    • Short Description: A Rabbit Polyclonal Antibody to SLC41A2 Transporter
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Solute carrier family 41 member 2, Solute carrier family 41 (magnesium transporter), Member 2
  • Anti-NIPAL4 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Magnesium Transporter NIPA4 
    • Product Code: ANT-144
    • Short Description: A Rabbit Polyclonal Antibody to Magnesium Transporter NIPA4 
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot
    • Target: Magnesium transporter NIPA4, NIPA-like protein 4, NIPA-like domain containing 4, Ichthyin, ICHYN, ICHN, Non-imprinted in Prader-Willi/Angelman syndrome region protein 4
  • Anti-NIPA2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Magnesium Transporter NIPA2
    • Product Code: ANT-142
    • Short Description: A Rabbit Polyclonal Antibody to Magnesium Transporter NIPA2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: Magnesium transporter NIPA2, Non-imprinted in Prader-Willi/Angelman syndrome region protein 2 homolog
  • Anti-xCT/SLC7A11 (extracellular)-mFluor™ Violet 450 Antibody
    A Rabbit Polyclonal Antibody to xCT/SLC7A11 (extracellular) conjugated to the fluorescent dye mFluor™ Violet 450.
    • Product Code: ANT-111-V
    • Short Description: A Rabbit Polyclonal Antibody to xCT/SLC7A11 (extracellular) conjugated to the fluorescent dye mFluor™ Violet 450.
    • Host: Rabbit
    • Label: mFluor™ Violet 450
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Slc7a11
  • Anti-xCT/SLC7A11 (extracellular)-PE Antibody
    A Rabbit Polyclonal Antibody to xCT/SLC7A11 Conjugated to the Fluorescent Dye R-Phycoerythrin
    • Product Code: ANT-111-PE
    • Short Description: A Rabbit Polyclonal Antibody to xCT/SLC7A11 Conjugated to the Fluorescent Dye R-Phycoerythrin
    • Host: Rabbit
    • Label: R-PE
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: Cystine/glutamate transporter, Amino acid transport system xc-, Solute carrier family 7 member 11, Calcium channel blocker resistance protein CCBR1
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