Protocols
The protocols below are useful guides on how to work with Alomone Labs’ products. For best results, we highly recommend that you calibrate certain experimental setups.
The protocols below are useful guides on how to work with Alomone Labs’ products. For best results, we highly recommend that you calibrate certain experimental setups.
Blocking Peptides Protocols
Blocking Peptide Protocol for Western Blot (WB)
Ensure primary antibody specificity in western blotting with a simple blocking peptide control.
Blocking Peptide Protocols for Immunohistochemistry (IHC) and Immunocytochemistry (ICC)
Improve antibody validation in IHC and ICC with a simple blocking peptide control.
Blocking Peptides – Controls for Better Results
Blocking peptides are one of several controls you should use to gain more insight into antibody specificity.
Toxin-Based Protocols
Toxin-Based Live-Cell Imaging Using Conjugated Toxins
Conjugated venom-derived peptide toxins serve as precise probes for live-cell imaging and biodetection.
Toxin-Based Live-Cell Flow Cytometry Using Conjugated Toxins
Live-cell flow cytometry with fluorophore- or biotin-conjugated venom-derived peptide toxins enables quantitative analysis of membrane proteins in living cells.
Toxin Histochemistry (THC) On Frozen Sections Using Conjugated Toxins
Venom-derived peptide toxins conjugated to fluorophores or biotin markers are powerful tools for specific biodetection in tissue sections.
Multiplex Immunodetection Protocol
Suitable for Frozen Sections Using Conjugated Toxins and Unconjugated Antibodies Directed Against Extracellular Epitopes.
Neurotrophin-Based Protocols
Immunofluorescence of Frozen Sections Using Conjugated Neurotrophins
Neurotrophins conjugated to fluorophores or biotin are valuable tools for the specific detection of target receptors in tissue sections.
Conjugated Neurotrophin-based Live-cell Flow Cytometry
Live-cell flow cytometry using fluorophore- or biotin-conjugated neurotrophins enables quantitative analysis of their receptors in living cells.
Live-Cell Imaging Using Conjugated Neurotrophins
Conjugated neurotrophins serve as selective probes for live-cell imaging and biodetection.
Immunohistochemistry Protocols
Immunohistochemistry (IHC) Protocols for Frozen Sections: Multiplex Staining
Target multiple proteins simultaneously with these multiplex IHC protocols using antibodies from the same or different host species.
Immunohistochemistry (IHC) Protocol for Frozen Heart Sections
Clear and detailed IHC steps for working with heart sections, from tissue preparation to staining.
Immunohistochemistry (IHC) Protocols for Frozen Sections: Direct Methods
A detailed protocol to take you through tissue processing and on to the direct method of IHC for floating sections using a fluorescent microscope.
Immunohistochemistry (IHC) Protocols for Frozen Sections: Indirect Methods
Detailed protocols to take you through tissue processing and on to indirect IHC methods for floating sections.
Immunohistochemistry (IHC) Protocols for Paraffin-embedded Sections
A clear protocol for using paraffin-embedded section with either DAB or fluorescence.
Sample Preparation Protocols
Sample Preparation from Tissues
Tissue homogenization and extraction for western blot analysis using denaturing or mild lysis buffers.
Sample Preparation from Cell Lines
Cultured cells sample preparation for western blot using denaturing extraction buffer or mild detergents.
Western Blot Protocols
Western Blot (WB) Protocol
A complete workflow for protein denaturation, separation, transfer, and antibody-based detection.
Western Blot (WB) Protocol for High Molecular Weight and Low-Abundance Proteins
An optimized workflow for resolving and detecting challenging protein targets.
XenoBlot™ Reconstitution Protocol for Western Blot
Confirm your western blot setup works exactly as intended with XenoBlot™, a ready-to-use validated positive control.
Immunocytochemistry Protocol
Immunocytochemistry (ICC) Protocols for Fixed or Live Cells: Indirect and Direct Methods
Detailed cell preparation and direct and indirect ICC methods.
Immunoprecipitation Protocol
Immunoprecipitation Using Dynabeads®
Straightforward immunoprecipitation with Dynabeads® and a denaturing elution.
Flow Cytometry Protocol
Flow Cytometry Protocols for Live Cells: Indirect and Direct Methods
Detailed steps to take you through cell preparation and on to indirect and direct methods of flow cytometry.