Cell Surface-Binding Antibodies

Cell Surface-Binding Antibodies

Antibodies targeting extracellular epitopes for surface labeling and live-cell detection

Cell surface-binding antibodies are essential tools for studying membrane proteins in their native, physiological context. These in-house produced antibodies recognize extracellular epitopes of cell surface proteins, enabling live-cell labeling, flow cytometry, surface receptor tracking, and functional studies without the need for cell permeabilization.
Alomone Labs offers a comprehensive portfolio of highly specific, well-validated antibodies targeting extracellular domains of ion channels, receptors, and transporters. These antibodies are carefully designed to ensure epitope accessibility, specificity, and reproducibility across advanced imaging and cytometry workflows. Each antibody is rigorously validated in immunohistochemistry (IHC), immunocytochemistry (ICC), western blot (WB), and/or flow cytometry, with matched blocking peptides available to confirm specificity.

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FAQs

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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803 Results
  • Anti-Tetraspanin 2 (extracellular) Antibody
    Anti-Tetraspanin 2 (extracellular) Antibody
    A Rabbit Polyclonal antibody to Tetraspanin 2 (extracellular)
    • Product Code: ANR-188
    • Short Description: A Rabbit Polyclonal antibody to Tetraspanin 2 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Tetraspan NET-3, TSPAN2
  • Anti-Tetraspanin 7 (extracellular) Antibody
    Anti-Tetraspanin 7 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Tetraspanin 7 (extracellular)
    • Product Code: ANR-187
    • Short Description: A Rabbit Polyclonal Antibody to Tetraspanin 7 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: TSPAN7, Transmembrane 4 Superfamily Member 2, TM4SF2, T-Cell Acute Lymphoblastic Leukemia-Associated Antigen 1, TALLA-1, Cell Surface Glycoprotein A15, A15, CD231.
  • Anti-Tetraspanin-3 (extracellular) Antibody
    Anti-Tetraspanin-3 (extracellular) Antibody
    A Rabbit Polyclonal antibody to Tetraspanin-3 (extracellular)
    • Product Code: ANR-185
    • Short Description: A Rabbit Polyclonal antibody to Tetraspanin-3 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: TSPAN3, Transmembrane 4 Superfamily Member 8, TM4SF8
  • Anti-Robo3 (extracellular) Antibody
    Anti-Robo3 (extracellular) Antibody
    A Rabbit Polyclonal antibody to Robo3 (extracellular)
    • Product Code: ANR-183
    • Short Description: A Rabbit Polyclonal antibody to Robo3 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Roundabout Homolog 3, Roundabout Guidance Receptor 3, Retinoblastoma-inhibiting gene 1 protein, RIG1
  • Anti-Robo2 (extracellular) Antibody
    Anti-Robo2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Robo2
    • Product Code: ANR-182
    • Short Description: A Rabbit Polyclonal Antibody to Robo2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot
    • Target: Roundabout homolog 2, Roundabout Guidance Receptor 2
  • Anti-Robo1 (extracellular)-FITC Antibody
    Anti-Robo1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Robo1 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ANR-181-F
    • Short Description: A Rabbit Polyclonal Antibody to Robo1 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Roundabout Guidance Receptor 1, Roundabout Homolog 1, Deleted In U Twenty Twenty, DUTT1
  • Anti-Robo1 (extracellular) Antibody
    Anti-Robo1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Robo1
    • Product Code: ANR-181
    • Short Description: A Rabbit Polyclonal Antibody to Robo1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Roundabout Guidance Receptor 1, Roundabout Homolog 1, Deleted In U Twenty Twenty, DUTT1
  • Anti-TMEM106B (extracellular) Antibody
    Anti-TMEM106B (extracellular) Antibody
    A Rabbit Polyclonal antibody to TMEM106B (extracellular)
    • Product Code: ANR-177
    • Short Description: A Rabbit Polyclonal antibody to TMEM106B (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Transmembrane Protein 106B
  • Anti-RAGE (extracellular) Antibody
    Anti-RAGE (extracellular) Antibody
    A Rabbit Polyclonal Antibody to RAGE
    • Product Code: ANR-176
    • Short Description: A Rabbit Polyclonal Antibody to RAGE
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot
    • Target: Advanced Glycosylation End Product-Specific Receptor, AGER, Receptor For Advanced Glycosylation End Products, SCARJ1
  • Anti-TMEM119 (extracellular)-PE Antibody
    Anti-TMEM119 (extracellular)-PE Antibody
    A Rabbit Polyclonal Antibody to TMEM119 conjugatd to R-Phycoerythrin (R-PE) fluorophore
    • Product Code: ANR-175-PE
    • Short Description: A Rabbit Polyclonal Antibody to TMEM119 conjugatd to R-Phycoerythrin (R-PE) fluorophore
    • Host: Rabbit
    • Label: R-PE
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Transmembrane Protein 119, Osteoblast induction factor, OBIF
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