Cell Surface-Binding Antibodies

Cell Surface-Binding Antibodies

Antibodies targeting extracellular epitopes for surface labeling and live-cell detection

Cell surface-binding antibodies are essential tools for studying membrane proteins in their native, physiological context. These in-house produced antibodies recognize extracellular epitopes of cell surface proteins, enabling live-cell labeling, flow cytometry, surface receptor tracking, and functional studies without the need for cell permeabilization.
Alomone Labs offers a comprehensive portfolio of highly specific, well-validated antibodies targeting extracellular domains of ion channels, receptors, and transporters. These antibodies are carefully designed to ensure epitope accessibility, specificity, and reproducibility across advanced imaging and cytometry workflows. Each antibody is rigorously validated in immunohistochemistry (IHC), immunocytochemistry (ICC), western blot (WB), and/or flow cytometry, with matched blocking peptides available to confirm specificity.

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FAQs

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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803 Results
  • Anti-Human TRPV6 (extracellular) Antibody
    Anti-Human TRPV6 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Human TRPV6 Channel
    • Product Code: ACC-028
    • Short Description: A Rabbit Polyclonal Antibody to Human TRPV6 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: Epithelial calcium channel 2, ECaC2, Calcium transport protein 1, CaT1, CaT-like, CaT-L, Transient receptor potential cation channel subfamily V member 6
  • Anti-CACNA2D1 (Ca<sub>V</sub>α2δ1) (extracellular) Antibody
    KO Validate'
    Anti-CACNA2D1 (CaVα2δ1) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to CACNA2D1 (CaVα2δ1 Subunit)
    • Product Code: ACC-015
    • Short Description: A Rabbit Polyclonal Antibody to CACNA2D1 (CaVα2δ1 Subunit)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: Voltage-dependent Ca2+ channel subunit alpha-2/delta-1, L-type calcium channel alpha 2 polypeptide, Dihydropyridine-sensitive L-type calcium channel alpha-2/delta subunit
  • Anti-BMPR1A (extracellular) Antibody
    Anti-BMPR1A (extracellular) Antibody
    A Rabbit Polyclonal antibody to BMPR1A (extracellular)
    • Product Code: ABR-043
    • Short Description: A Rabbit Polyclonal antibody to BMPR1A (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Bone Morphogenetic Protein Receptor Type-1A, Activin Receptor-Like Kinase 3, ALK3, Serine/Threonine-Protein Kinase Receptor R5, SKR5, CD292
  • Anti-ACVRL1 (extracellular) Antibody
    Anti-ACVRL1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to ACVRL1
    • Product Code: ABR-041
    • Short Description: A Rabbit Polyclonal Antibody to ACVRL1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: Activin A Receptor Like Type 1, ALK1, Serine/Threonine-Protein Kinase Receptor R3, SKR3, TGF-B Superfamily Receptor Type I, TSR-I
  • Anti-BAI2 (extracellular) Antibody
    Anti-BAI2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to BAI2
    • Product Code: ABR-022
    • Short Description: A Rabbit Polyclonal Antibody to BAI2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Brain-specific angiogenesis inhibitor 2, Adhesion G protein-coupled receptor B2, ADGRB2
  • Anti-BAI1 (extracellular)-FITC Antibody
    Anti-BAI1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to BAI1 Conjugated to the Fluorescent Dye FITC
    • Product Code: ABR-021-F
    • Short Description: A Rabbit Polyclonal Antibody to BAI1 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Brain-specific angiogenesis inhibitor 1, Adhesion G protein-coupled receptor B1, ADGRB1, GDAIF
  • Anti-BAI1 (extracellular) Antibody
    Anti-BAI1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to BAI1
    • Product Code: ABR-021
    • Short Description: A Rabbit Polyclonal Antibody to BAI1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Brain-specific angiogenesis inhibitor 1, Adhesion G protein-coupled receptor B1, ADGRB1, GDAIF
  • Anti-B1 Bradykinin Receptor (BDKRB1) (extracellular)-FITC Antibody
    Anti-B1 Bradykinin Receptor (BDKRB1) (extracellular)-FITC Antibody
    A Rabbit Polyclonal antibody to Human BDKRB1(extracellular) conjugated to FITC fluorophore.
    • Product Code: ABR-013-F
    • Short Description: A Rabbit Polyclonal antibody to Human BDKRB1(extracellular) conjugated to FITC fluorophore.
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: BK-1 receptor, BKRB1, B1R, BKR2
  • Anti-B1 Bradykinin Receptor (BDKRB1) (extracellular) Antibody
    Anti-B1 Bradykinin Receptor (BDKRB1) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to B1 Bradykinin Receptor
    • Product Code: ABR-013
    • Short Description: A Rabbit Polyclonal Antibody to B1 Bradykinin Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: BK-1 receptor, BKRB1, B1R, BKR1
  • Anti-Bombesin Receptor 3 (extracellular) Antibody
    Anti-Bombesin Receptor 3 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Bombesin Receptor 3
    • Product Code: ABR-003
    • Short Description: A Rabbit Polyclonal Antibody to Bombesin Receptor 3
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: BB3R, BRS3
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