Cell Surface

Cell Surface

Antibodies targeting extracellular epitopes for surface labeling and live-cell detection

Cell surface-binding antibodies are essential tools for studying membrane proteins in their native, physiological context. These in-house produced antibodies recognize extracellular epitopes of cell surface proteins, enabling live-cell labeling, flow cytometry, surface receptor tracking, and functional studies without the need for cell permeabilization.
Alomone Labs offers a comprehensive portfolio of highly specific, well-validated antibodies targeting extracellular domains of ion channels, receptors, and transporters. These antibodies are carefully designed to ensure epitope accessibility, specificity, and reproducibility across advanced imaging and cytometry workflows. Each antibody is rigorously validated in immunohistochemistry (IHC), immunocytochemistry (ICC), western blot (WB), and/or flow cytometry, with matched blocking peptides available to confirm specificity.

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FAQs

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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803 Results
  • Anti-FSHR (extracellular) Antibody
    Anti-FSHR (extracellular) Antibody
    A Rabbit Polyclonal Antibody to FSH Receptor
    • Product Code: AFR-010
    • Short Description: A Rabbit Polyclonal Antibody to FSH Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: FSH receptor, Follicle-stimulating hormone receptor, Follitropin receptor, FSHRO
  • Anti-Human FPR2/ALX (extracellular) Antibody
    Anti-Human FPR2/ALX (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Human FPR2/ALX
    • Product Code: AFR-002
    • Short Description: A Rabbit Polyclonal Antibody to Human FPR2/ALX
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: N-formyl peptide receptor 2, FMLP-related receptor I, FMLPX, Formyl peptide receptor-like 1, FPRL1, ALXR, Lipoxin A4 receptor, LXA4 receptor, LXA4R, RFP
  • Anti-Human FPR1 (extracellular)-ATTO Fluor-488 Antibody
    Anti-Human FPR1 (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to FPR1 Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: AFR-001-AG
    • Short Description: A Rabbit Polyclonal Antibody to FPR1 Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human
    • Applications: Live cell imaging, Flow cytometry
    • Target: N-formyl peptide receptor 1, fMLP receptor
  • Anti-Human FPR1 (extracellular) Antibody
    Anti-Human FPR1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Human FPR1
    • Product Code: AFR-001
    • Short Description: A Rabbit Polyclonal Antibody to Human FPR1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: N-formyl peptide receptor 1, fMLP receptor
  • Anti-Human CD97/ADGRE5 (extracellular) Antibody
    Anti-Human CD97/ADGRE5 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to CD97/ADGRE5
    • Product Code: AER-055
    • Short Description: A Rabbit Polyclonal Antibody to CD97/ADGRE5
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Western blot
    • Target: Adhesion G protein-coupled receptor E5, Leukocyte antigen CD97, Seven-transmembrane heterodimeric receptor associated with inflammation
  • Guinea pig Anti-EMR1 (ADGRE1) (extracellular) Antibody
    Guinea pig Anti-EMR1 (ADGRE1) (extracellular) Antibody
    A Guinea Pig Polyclonal Antibody to EMR1
    • Product Code: AER-051-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to EMR1
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: MR1 hormone receptor, EGF-like module-containing mucin-like hormone receptor-like 1, Adhesion G protein-coupled receptor E1, TM7LN3, F4/80, GPF480
  • Anti-EMR1 (ADGRE1) (extracellular)-FITC Antibody
    Anti-EMR1 (ADGRE1) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to EMR1 Conjugated to the Fluorescent Dye FITC
    • Product Code: AER-051-F
    • Short Description: A Rabbit Polyclonal Antibody to EMR1 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: EMR1 hormone receptor, EGF-like module-containing mucin-like hormone receptor-like 1, Adhesion G protein-coupled receptor E1, TM7LN3, F4/80, GPF480
  • Anti-EMR1 (ADGRE1) (extracellular) Antibody
    Anti-EMR1 (ADGRE1) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to EMR1
    • Product Code: AER-051
    • Short Description: A Rabbit Polyclonal Antibody to EMR1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: EMR1 hormone receptor, EGF-like module-containing mucin-like hormone receptor-like 1, Adhesion G protein-coupled receptor E1, TM7LN3, F4/80, GPF480
  • Anti-GPER1/GPR30 (extracellular)-FITC Antibody
    Anti-GPER1/GPR30 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to GPER1 Conjugated to the Fluorescent Dye FITC
    • Product Code: AER-049-F
    • Short Description: A Rabbit Polyclonal Antibody to GPER1 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: G Protein-Coupled Estrogen Receptor 1, G Protein-Coupled Receptor 30, Membrane Estrogen Receptor, Chemoattractant Receptor-Like 2, GPER
  • Anti-GPER1/GPR30 (extracellular) Antibody-APC Antibody
    Anti-GPER1/GPR30 (extracellular) Antibody-APC Antibody
    A Rabbit Polyclonal Antibody to GPER1 (extracellular) conjugated to Allophycocyanin (APC) fluorophore.
    • Product Code: AER-049-APC
    • Short Description: A Rabbit Polyclonal Antibody to GPER1 (extracellular) conjugated to Allophycocyanin (APC) fluorophore.
    • Host: Rabbit
    • Label: APC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: GPER1
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