Flow Cytometry Antibodies

Flow Cytometry Antibodies

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

779 Results
  • Anti-Na<sub>V</sub>1.8 (SCN10A) (extracellular) Antibody
    Anti-NaV1.8 (SCN10A) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to NaV1.8 (SCN10A) Channel
    • Product Code: ASC-028
    • Short Description: A Rabbit Polyclonal Antibody to NaV1.8 (SCN10A) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: PN3, SNS, Sodium channel protein type 10 subunit alpha
  • Anti-SCN3A (Na<sub>V</sub>1.3) (extracellular) Antibody
    Anti-SCN3A (NaV1.3) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to SCN3A (NaV1.3) Channel
    • Product Code: ASC-023
    • Short Description: A Rabbit Polyclonal Antibody to SCN3A (NaV1.3) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: BIII, Brain type III Na+ channel, Sodium channel protein type 3 subunit alpha
  • Anti-NALCN/VGCNL1 (extracellular) Antibody
    Anti-NALCN/VGCNL1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Sodium Leak Channel Non-Selective Protein (NALCN)
    • Product Code: ASC-022
    • Short Description: A Rabbit Polyclonal Antibody to Sodium Leak Channel Non-Selective Protein (NALCN)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Sodium leak channel non-selective protein, Voltage gated channel-like protein 1, Four domain-type voltage-gated ion channel alpha-1 subunit, CanIon, Rb21-channel
  • Anti-ENaC β (SCNN1B) (extracellular) Antibody
    Anti-ENaC β (SCNN1B) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to ENaCβ Channel
    • Product Code: ASC-019
    • Short Description: A Rabbit Polyclonal Antibody to ENaCβ Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Amiloride-sensitive sodium channel subunit beta, Beta-NaCH, Epithelial sodium channel β subunit, Beta-ENaC, Nonvoltage-gated sodium channel 1 subunit beta, SCNEB
  • Anti-ENaC γ (SCNN1G) (extracellular) Antibody
    Anti-ENaC γ (SCNN1G) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to ENaC? Channel
    • Product Code: ASC-011
    • Short Description: A Rabbit Polyclonal Antibody to ENaC? Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Amiloride-sensitive sodium channel subunit gamma, Epithelial sodium channel γ subunit, Gamma-ENaC, Gamma-NaCH, Nonvoltage-gated sodium channel 1 subunit gamma, SCNEG
  • Anti-RAMP2 (extracellular) Antibody
    Anti-RAMP2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to RAMP2
    • Product Code: ARR-022
    • Short Description: A Rabbit Polyclonal Antibody to RAMP2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: Receptor activity-modifying protein 2, CRLR activity-modifying protein 2, Calcitonin receptor-like receptor activity-modifying protein 2
  • Anti-RAMP1 (extracellular) Antibody
    KO Validate'
    Anti-RAMP1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to RAMP1
    • Product Code: ARR-021
    • Short Description: A Rabbit Polyclonal Antibody to RAMP1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Receptor activity-modifying protein 1, Calcitonin receptor-like receptor activity-modifying protein 1
  • Anti-RXFP2 (extracellular) Antibody
    Anti-RXFP2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Relaxin Receptor 2
    • Product Code: ARR-012
    • Short Description: A Rabbit Polyclonal Antibody to Relaxin Receptor 2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: Relaxin receptor 2, G-protein coupled receptor affecting testicular descent, Leucine-rich repeat-containing G-protein coupled receptor 8, GPR106, GREAT, LGR8, INSL3R
  • Anti-Aquaporin 11 (extracellular) Antibody
    Anti-Aquaporin 11 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Aquaporin 11 Channel
    • Product Code: AQP-011
    • Short Description: A Rabbit Polyclonal Antibody to Aquaporin 11 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: AQP11
  • Anti-PepT2/SLC15A2 (extracellular) Antibody
    Anti-PepT2/SLC15A2 (extracellular) Antibody
    A Rabbit Polyclonal antibody to PepT2/SLC15A2 (extracellular)
    • Product Code: APT-002
    • Short Description: A Rabbit Polyclonal antibody to PepT2/SLC15A2 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Peptide Transporter 2, Solute Carrier Family 15 Member 2, Oligopeptide Transporter - Kidney Isoform, Kidney H+/Peptide Cotransporter
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