Flow Cytometry Antibodies

Flow Cytometry Antibodies

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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801 Results
  • Anti-Piezo1 (extracellular)-ATTO Fluor-550 Antibody
    Anti-Piezo1 (extracellular)-ATTO Fluor-550 Antibody
    A Rabbit Polyclonal Antibody to Piezo1 (extracellular) conjugated to the fluorescent dye ATTO Fluor-550.
    • Product Code: APC-087-AO
    • Short Description: A Rabbit Polyclonal Antibody to Piezo1 (extracellular) conjugated to the fluorescent dye ATTO Fluor-550.
    • Host: Rabbit
    • Label: ATTO 550
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry, Immunohistochemistry
    • Target: Piezo1
  • Anti-Piezo1 (extracellular) Antibody
    KO Validate'
    Anti-Piezo1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Piezo1 Channel
    • Product Code: APC-087
    • Short Description: A Rabbit Polyclonal Antibody to Piezo1 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Piezo-type mechanosensitive ion channel component 1, Protein FAM38A, Mib, Membrane protein induced by beta-amyloid treatment
  • Anti-HCN3 (extracellular) Antibody
    Anti-HCN3 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to HCN3 Channel
    • Product Code: APC-083
    • Short Description: A Rabbit Polyclonal Antibody to HCN3 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: K+/Na+ Hyperpolarization-activated cyclic nucleotide-gated channel 3, BCNG4, Hac3
  • Anti-<em>slo</em>β3 (KCNMB3) (extracellular) Antibody
    Anti-sloβ3 (KCNMB3) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to slo?3 (KCNMB3) Subunit
    • Product Code: APC-068
    • Short Description: A Rabbit Polyclonal Antibody to slo?3 (KCNMB3) Subunit
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Ca2+-activated K+ channel subunit β3, BKCa β3, BK beta 3, Maxi K channel β3, Charybdotoxin receptor subunit beta-3
  • Anti-KCNK9 (TASK-3) (extracellular) Antibody
    Anti-KCNK9 (TASK-3) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to KCNK9 Channel 
    • Product Code: APC-044
    • Short Description: A Rabbit Polyclonal Antibody to KCNK9 Channel 
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Potassium channel subfamily K member 9, K2P9.1, TWIK-related acid-sensitive K+ channel 3, Two pore K+ channel KT3.2
  • Anti-Opsin 3 (extracellular) Antibody
    Anti-Opsin 3 (extracellular) Antibody
    A Rabbit Polyclonal antibody to Opsin 3 (extracellular)
    • Product Code: AOR-023
    • Short Description: A Rabbit Polyclonal antibody to Opsin 3 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: OPN3, Encephalopsin, Panopsin
  • Anti-δ-Opioid Receptor (OPRD1) (extracellular) Antibody
    Anti-δ-Opioid Receptor (OPRD1) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to ?-Opioid Receptor (OPRD1)
    • Product Code: AOR-014
    • Short Description: A Rabbit Polyclonal Antibody to ?-Opioid Receptor (OPRD1)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: DOR-1
  • Anti-κ-Opioid Receptor (OPRK1) (extracellular)-FITC Antibody
    Anti-κ-Opioid Receptor (OPRK1) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to ?-Opioid Receptor (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: AOR-012-F
    • Short Description: A Rabbit Polyclonal Antibody to ?-Opioid Receptor (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Kappa-type opioid receptor, KOR-1, KOR, Opiate receptor kappa 1
  • Anti-κ-Opioid Receptor (OPRK1) (extracellular) Antibody
    Anti-κ-Opioid Receptor (OPRK1) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to ?-Opioid Receptor (OPRK1)
    • Product Code: AOR-012
    • Short Description: A Rabbit Polyclonal Antibody to ?-Opioid Receptor (OPRK1)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Kappa-type opioid receptor, KOR-1, Opiate receptor kappa 1
  • Anti-µ-Opioid Receptor/OPRM1 (extracellular)-FITC Antibody
    Anti-µ-Opioid Receptor/OPRM1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to OPRM1 Conjugated to the Fluorescent Dye FITC
    • Product Code: AOR-011-F
    • Short Description: A Rabbit Polyclonal Antibody to OPRM1 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Mu-type opioid receptor, Opioid receptor B, MOR-1, MOP, Mu-opiate receptor
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