Flow Cytometry Antibodies

Flow Cytometry Antibodies

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FAQs

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.
  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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801 Results
  • Anti-LRRTM1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to LRRTM1
    • Product Code: ANR-141
    • Short Description: A Rabbit Polyclonal Antibody to LRRTM1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Leucine-rich repeat transmembrane neuronal protein 1
  • Anti-SORCS2 (extracellular) Antibody
    A Rabbit Polyclonal antibody to SORCS2
    • Product Code: ANR-135
    • Short Description: A Rabbit Polyclonal antibody to SORCS2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Sortilin Related VPS10 Domain Containing Receptor 2, VPS10 Domain-Containing Receptor SorCS2
  • Anti-SORCS1 (extracellular) Antibody
    A Rabbit Polyclonal antibody to SORCS1
    • Product Code: ANR-134
    • Short Description: A Rabbit Polyclonal antibody to SORCS1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Sortilin-Related VPS10 Domain Containing Receptor 1, VPS10 Domain-Containing Receptor SorCS1
  • Anti-SORL1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to SORL1 Receptor
    • Product Code: ANR-132
    • Short Description: A Rabbit Polyclonal Antibody to SORL1 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot, Immunohistochemistry
    • Target: Sortilin Related Receptor 1, Low-Density Lipoprotein Receptor relative with 11 Ligand-Binding Repeats, LR11, Sorting Protein-Related Receptor Containing LDLR Class A Repeats, SORLA
  • Anti-RGMA (extracellular) Antibody
    A Rabbit polyclonal antibody to RGMA (extracellular).
    • Product Code: ANR-126
    • Short Description: A Rabbit polyclonal antibody to RGMA (extracellular).
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Repulsive Guidance Molecule A, RGM Domain Family Member A, Repulsive Guidance Molecule BMP Co-Receptor A, RGMa
  • Anti-ADAM22 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to ADAM22
    • Product Code: ANR-120
    • Short Description: A Rabbit Polyclonal Antibody to ADAM22
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Disintegrin and metalloproteinase domain-containing protein 22, ADAM metallopeptidase domain 22, MDC2
  • Anti-NRG3 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Neuregulin-3
    • Product Code: ANR-115
    • Short Description: A Rabbit Polyclonal Antibody to Neuregulin-3
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Neuregulin-3, HRG3
  • Anti-NRG2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Neuregulin-2
    • Product Code: ANR-114
    • Short Description: A Rabbit Polyclonal Antibody to Neuregulin-2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Neuregulin-2, Pro-neuregulin-2 membrane-bound isoform, Pro-NRG2, Neural- and thymus-derived activator for ErbB kinases, NTAK
  • Anti-NRG1 Type III (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Neuregulin-1 Type III
    • Product Code: ANR-113
    • Short Description: A Rabbit Polyclonal Antibody to Neuregulin-1 Type III
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Neuregulin-1 isoform 10, CRD-NRG, Sensory and motor neuron derived factor, SMDF
  • Anti-NRG1 (Neuregulin-1) (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to Neuregulin-1 Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: ANR-111-AG
    • Short Description: A Rabbit Polyclonal Antibody to Neuregulin-1 Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: Glial growth factor, GGF, Heregulin, HRG, Sensory and motor neuron derived factor, SMDF, Neu differentiation factor, NDF
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