Flow Cytometry Antibodies

Flow Cytometry Antibodies

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FAQs

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.
  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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803 Results
  • Anti-GLUT1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Glucose Transporter 1 Conjugated to the Fluorescent Dye FITC
    • Product Code: AGT-041-F
    • Short Description: A Rabbit Polyclonal Antibody to Glucose Transporter 1 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: Glucose transporter 1, Facilitated glucose transporter member 1, SLC2A1
  • Anti-GLUT1 (extracellular)-APC Antibody
    A Rabbit Polyclonal Antibody to GLUT1 (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)
    • Product Code: AGT-041-APC
    • Short Description: A Rabbit Polyclonal Antibody to GLUT1 (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)
    • Host: Rabbit
    • Label: APC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Glucose transporter 1, Facilitated glucose transporter member 1, SLC2A1, DYT9
  • Anti-GLUT1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Glucose Transporter 1
    • Product Code: AGT-041
    • Short Description: A Rabbit Polyclonal Antibody to Glucose Transporter 1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot
    • Target: Glucose transporter 1, Facilitated glucose transporter member 1, SLC2A1
  • Anti-SGLT2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to SGLT2 Transporter
    • Product Code: AGT-032
    • Short Description: A Rabbit Polyclonal Antibody to SGLT2 Transporter
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Na+/glucose cotransporter 2, Low affinity sodium-glucose cotransporter, SLC5A2
  • Anti-SGLT1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to SGLT1 Transporter
    • Product Code: AGT-031
    • Short Description: A Rabbit Polyclonal Antibody to SGLT1 Transporter
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Na+/glucose cotransporter 1, NAGT, High affinity sodium-glucose cotransporter, SLC5A1
  • Anti-GLUT8 (extracellular) Antibody
    A Rabbit Polyclonal antibody to GLUT8 (extracellular)
    • Product Code: AGT-028
    • Short Description: A Rabbit Polyclonal antibody to GLUT8 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Glucose Transporter Type 8, Glucose Transporter Type X1, GLUTX1, Solute Carrier Family 2 Member 8, SLC2A8
  • Anti-GLUT3 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Glucose Transporter 3 Conjugated to the Fluorescent Dye FITC
    • Product Code: AGT-023-F
    • Short Description: A Rabbit Polyclonal Antibody to Glucose Transporter 3 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Rat, Mouse
    • Applications: Flow cytometry
    • Target: Glucose transporter 3, Facilitated glucose transporter member 3, SLC2A3
  • Anti-GLUT3 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Glucose Transporter 3
    • Product Code: AGT-023
    • Short Description: A Rabbit Polyclonal Antibody to Glucose Transporter 3
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Glucose transporter 3, Facilitated glucose transporter member 3, SLC2A3
  • Anti-SLC6A14 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to SLC6A14 Transporter
    • Product Code: AGT-015
    • Short Description: A Rabbit Polyclonal Antibody to SLC6A14 Transporter
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Sodium and chloride-dependent neutral and basic amino acid transporter B(0+), Amino acid transporter ATB0+, Solute carrier family 6 member 14
  • Anti-GlyT2 (SLC6A5) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Glycine Transporter 2 (SLC6A5)
    • Product Code: AGT-012
    • Short Description: A Rabbit Polyclonal Antibody to Glycine Transporter 2 (SLC6A5)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Na+/Cl--dependent glycine transporter 2
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