Multiplex Immunodetection

Multiplex Immunodetection

Maximize Targets, Minimize Cross-Reactivity

We offer in-house–produced and thoroughly tested antibodies conjugated to a variety of fluorophores, as well as extracellular-epitope reagents optimized for multiplex immunofluorescence, immunocolocalization, and high-parameter imaging. Guinea pig–derived antibodies are also available, providing additional species flexibility to help researchers build highly specific panels with minimal cross-reactivity. All antibodies are rigorously validated for applications including immunohistochemistry (IHC), immunocytochemistry (ICC), western blot (WB), and flow cytometry.

Shop Popular Products

Product Categories

No categories available

FAQs

Lorem ipsum dolor sit amet, consectetur adipiscing elit. Curabitur quis risus id enim vehicula interdum et ac sem.

  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

322 Results
  • Anti-Neuropeptide S Receptor (extracellular)-FITC Antibody
    Anti-Neuropeptide S Receptor (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Neuropeptide S Receptor (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ANR-029-F
    • Short Description: A Rabbit Polyclonal Antibody to Neuropeptide S Receptor (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: NPSR1, G-Protein Coupled Receptor 154, GPR154, G-Protein Coupled Receptor for Asthma Susceptibility, GPRA
  • Anti-Neuropilin-1 (NRP1) (extracellular)-FITC Antibody
    Anti-Neuropilin-1 (NRP1) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Neuropilin-1 (NRP1) (extracellular) conjugated to the fluorescent dye FITC.
    • Product Code: ANR-063-F
    • Short Description: A Rabbit Polyclonal Antibody to Neuropilin-1 (NRP1) (extracellular) conjugated to the fluorescent dye FITC.
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: Vascular endothelial cell growth factor 165 receptor, VEGF165R, CD304
  • Anti-Neuropilin-2 (NRP2) (extracellular)-FITC Antibody
    Anti-Neuropilin-2 (NRP2) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Neuropilin-2 Conjugated to the Fluorescent Dye FITC
    • Product Code: ANR-062-F
    • Short Description: A Rabbit Polyclonal Antibody to Neuropilin-2 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: Vascular endothelial cell growth factor 165 receptor 2, VEGF165R2
  • Anti-Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular)-ATTO Fluor-633 Antibody
    Anti-Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular)-ATTO Fluor-633 Antibody
    A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor α7 Conjugated to the Fluorescent Dye ATTO-633
    • Product Code: ANC-007-FR
    • Short Description: A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor α7 Conjugated to the Fluorescent Dye ATTO-633
    • Host: Rabbit
    • Label: ATTO 633
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: nAChR α7, Cholinergic receptor nicotinic alpha 7, Neuronal acetylcholine receptor subunit alpha-7, ACRA7
  • Anti-Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular)-FITC Antibody
    Anti-Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor α7 Conjugated to the Fluorescent Dye FITC
    • Product Code: ANC-007-F
    • Short Description: A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor α7 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: nAChR α7, Cholinergic receptor nicotinic alpha 7, Neuronal acetylcholine receptor subunit alpha-7, ACRA7
  • Anti-Nicotinic Acetylcholine Receptor β2 (CHRNB2) (extracellular)-ATTO Fluor-594 Antibody
    Anti-Nicotinic Acetylcholine Receptor β2 (CHRNB2) (extracellular)-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor β2 Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: ANC-012-AR
    • Short Description: A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor β2 Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: nAChR β2, Neuronal acetylcholine receptor subunit beta-2, Cholinergic receptor nicotinic beta 2, ACRB2, EFNL3
  • Anti-Nicotinic Acetylcholine Receptor β4 (CHRNB4) (extracellular)-ATTO Fluor-594 Antibody
    Anti-Nicotinic Acetylcholine Receptor β4 (CHRNB4) (extracellular)-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor β4 Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: ANC-014-AR
    • Short Description: A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor β4 Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: nAChR β4, Neuronal acetylcholine receptor subunit beta-4, Cholinergic receptor nicotinic beta 4
  • Anti-NMDAR2A (GluN2A) (extracellular)-ATTO Fluor-488 Antibody
    Anti-NMDAR2A (GluN2A) (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to NMDA Receptor 2A (GluN2A) Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: AGC-002-AG
    • Short Description: A Rabbit Polyclonal Antibody to NMDA Receptor 2A (GluN2A) Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: NMDA receptor 2A, NR2A, GRIN2A, Ionotropic glutamate receptor subunit ε1, N-methyl-D-aspartate receptor subunit 2A
  • Anti-NMDAR2B (GluN2B) (extracellular)-ATTO Fluor-594 Antibody
    Anti-NMDAR2B (GluN2B) (extracellular)-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to NMDA Receptor 2B (GluN2B) Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: AGC-003-AR
    • Short Description: A Rabbit Polyclonal Antibody to NMDA Receptor 2B (GluN2B) Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: NMDA receptor 2B, Ionotropic glutamate receptor subunit ε2, N-methyl-D-aspartate receptor subunit 2B, GRIN2B, NR2B
  • Anti-Nociceptin Receptor (OPRL1)-ATTO Fluor-594 Antibody
    Anti-Nociceptin Receptor (OPRL1)-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to Nociceptin Receptor Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: AOR-015-AR
    • Short Description: A Rabbit Polyclonal Antibody to Nociceptin Receptor Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: κ-Type 3 opioid receptor, KOR-3, Orphanin FQ receptor, ROR-C, XOR1, ORL1, N/OFQ receptor
Load More Results
Loading...

Have questions about our products?

Step-by-Step Protocols