Multiplex Immunodetection

Multiplex Immunodetection

Maximize Targets, Minimize Cross-Reactivity

We offer in-house–produced and thoroughly tested antibodies conjugated to a variety of fluorophores, as well as extracellular-epitope reagents optimized for multiplex immunofluorescence, immunocolocalization, and high-parameter imaging. Guinea pig–derived antibodies are also available, providing additional species flexibility to help researchers build highly specific panels with minimal cross-reactivity. All antibodies are rigorously validated for applications including immunohistochemistry (IHC), immunocytochemistry (ICC), western blot (WB), and flow cytometry.

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FAQs

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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322 Results
  • Anti-P2Y12 Receptor-ATTO Fluor-594 Antibody
    Anti-P2Y12 Receptor-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to P2Y12 Receptor Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: APR-012-AR
    • Short Description: A Rabbit Polyclonal Antibody to P2Y12 Receptor Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: P2Y purinoceptor 12, P2RY12, ADP-glucose receptor, Platelet ADP receptor, HORK3
  • Anti-P2Y13 Receptor (extracellular)-FITC Antibody
    Anti-P2Y13 Receptor (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to P2Y13 Receptor  (extracellular) conjugated to the fluorescent dye FITC 
    • Product Code: APR-103-F
    • Short Description: A Rabbit Polyclonal Antibody to P2Y13 Receptor  (extracellular) conjugated to the fluorescent dye FITC 
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Purinergic Receptor P2Y13, P2RY13, GPCR1, GPR86, GPR94, SP174
  • Anti-P2Y14 (extracellular)-FITC Antibody
    Anti-P2Y14 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to P2Y14 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: APR-018-F
    • Short Description: A Rabbit Polyclonal Antibody to P2Y14 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: P2Y purinoceptor 14, P2RY14, GPR105, UDP-glucose receptor
  • Anti-P2Y2 Receptor (extracellular)-FITC Antibody
    Anti-P2Y2 Receptor (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to P2Y2 Receptor Conjugated to the Fluorescent Dye FITC
    • Product Code: APR-102-F
    • Short Description: A Rabbit Polyclonal Antibody to P2Y2 Receptor Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: P2Y purinoceptor 2, P2RY2, P2U1
  • Anti-P2Y6 Receptor (extracellular)-FITC Antibody
    Anti-P2Y6 Receptor (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to P2Y6 Conjugated to the Fluorescent Dye FITC
    • Product Code: APR-106-F
    • Short Description: A Rabbit Polyclonal Antibody to P2Y6 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: P2Y purinoceptor 6, P2RY6
  • Anti-p75 NGF Receptor (extracellular)-ATTO Fluor-488 Antibody
    Anti-p75 NGF Receptor (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to Human Neurotrophin Receptor p75NTR Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: ANT-007-AG
    • Short Description: A Rabbit Polyclonal Antibody to Human Neurotrophin Receptor p75NTR Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Immunocytochemistry, Immunofluorescence, Flow cytometry, Immunohistochemistry
    • Target: p75NTR, p75 Neurotrophin receptor, Low affinity NGF receptor, TNFR superfamily member 16, TNFRSF16, CD271
  • Anti-p75 NGF Receptor (extracellular)-ATTO Fluor-550 Antibody
    Anti-p75 NGF Receptor (extracellular)-ATTO Fluor-550 Antibody
    A Rabbit Polyclonal Antibody to Human Neurotrophin Receptor p75NTR Conjugated to the Fluorescent Dye ATTO-550
    • Product Code: ANT-007-AO
    • Short Description: A Rabbit Polyclonal Antibody to Human Neurotrophin Receptor p75NTR Conjugated to the Fluorescent Dye ATTO-550
    • Host: Rabbit
    • Label: ATTO 550
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: p75NTR, p75 Neurotrophin receptor, Low affinity NGF receptor, TNFR superfamily member 16, TNFRSF16, CD271
  • Anti-p75 NGF Receptor (extracellular)-FITC Antibody
    Anti-p75 NGF Receptor (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Human Neurotrophin Receptor p75NTR Conjugated to the Fluorescent Dye FITC
    • Product Code: ANT-007-F
    • Short Description: A Rabbit Polyclonal Antibody to Human Neurotrophin Receptor p75NTR Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: p75NTR, p75 Neurotrophin receptor, Low affinity NGF receptor, TNFR superfamily member 16, TNFRSF16, CD271
  • Anti-PAR4 (F2RL3) (extracellular)-FITC Antibody
    KO Validate'
    Anti-PAR4 (F2RL3) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Protease-Activated Receptor-4 Conjugated to the Fluorescent Dye FITC
    • Product Code: APR-034-F
    • Short Description: A Rabbit Polyclonal Antibody to Protease-Activated Receptor-4 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Protease-activated receptor-4, PAR-4, Coagulation factor II receptor-like 3, Thrombin receptor-like 3
  • Anti-Piezo1 (extracellular)-FITC Antibody
    Anti-Piezo1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Piezo1 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: APC-087-F
    • Short Description: A Rabbit Polyclonal Antibody to Piezo1 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Piezo-type mechanosensitive ion channel component 1, Protein FAM38A, Mib, Membrane protein induced by beta-amyloid treatment
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