Multiplex Immunodetection

Multiplex Immunodetection

Maximize Targets, Minimize Cross-Reactivity

We offer in-house–produced and thoroughly tested antibodies conjugated to a variety of fluorophores, as well as extracellular-epitope reagents optimized for multiplex immunofluorescence, immunocolocalization, and high-parameter imaging. Guinea pig–derived antibodies are also available, providing additional species flexibility to help researchers build highly specific panels with minimal cross-reactivity. All antibodies are rigorously validated for applications including immunohistochemistry (IHC), immunocytochemistry (ICC), western blot (WB), and flow cytometry.

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FAQs

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.
  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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322 Results
  • Anti-Platelet-activating factor receptor (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Platelet-activating factor receptor (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: APR-055-F
    • Short Description: A Rabbit Polyclonal Antibody to Platelet-activating factor receptor (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: PTAFR, PAFR
  • Anti-Plexin-A1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Plexin-A1 Conjugated to the Fluorescent Dye FITC
    • Product Code: APR-081-F
    • Short Description: A Rabbit Polyclonal Antibody to Plexin-A1 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: PLXNA1, PLXN1, Semaphorin receptor NOV
  • Anti-Plexin-C1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to PLXNC1 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: APR-091-F
    • Short Description: A Rabbit Polyclonal Antibody to PLXNC1 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Virus-Encoded Semaphorin Protein Receptor, VESPR, CD232, PLXNC1
  • Anti-Presenilin-1-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to Presenilin-1 Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: AIP-011-AG
    • Short Description: A Rabbit Polyclonal Antibody to Presenilin-1 Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: PSEN1, PS-1, Psnl1
  • Anti-proBDNF-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to proBDNF Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: ANT-006-AG
    • Short Description: A Rabbit Polyclonal Antibody to proBDNF Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Flow cytometry, Immunohistochemistry
    • Target: Brain-derived neurotrophic factor precursor
  • Anti-Prostacyclin Receptor (PTGIR) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to PTGIR (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: APR-068-F
    • Short Description: A Rabbit Polyclonal Antibody to PTGIR (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Prostanoid IP receptor, Prostaglandin I2 receptor, PGI2 receptor, PRIPR
  • Anti-Prostaglandin E Receptor EP2/PTGER2 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Prostanoid EP2 Receptor Conjugated to the Fluorescent Dye FITC
    • Product Code: APR-064-F
    • Short Description: A Rabbit Polyclonal Antibody to Prostanoid EP2 Receptor Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Prostaglandin E2 receptor EP2 subtype, PGE2 receptor EP2 subtype, Prostanoid EP2 receptor
  • Anti-PTH2R (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to Parathyroid Hormone 2 Receptor Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: APR-052-AG
    • Short Description: A Rabbit Polyclonal Antibody to Parathyroid Hormone 2 Receptor Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: Parathyroid hormone 2 receptor, PTH2 receptor, PTHR2
  • Anti-Ret Receptor (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Ret receptor (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ANT-025-F
    • Short Description: A Rabbit Polyclonal Antibody to Ret receptor (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Proto-oncogene tyrosine-protein kinase receptor Ret, Proto-oncogene c-Ret, Cadherin family member 12, CDHF12, PTC, RET51
  • Anti-Robo1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Robo1 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ANR-181-F
    • Short Description: A Rabbit Polyclonal Antibody to Robo1 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Roundabout Guidance Receptor 1, Roundabout Homolog 1, Deleted In U Twenty Twenty, DUTT1
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