Multiplex Immunodetection

Multiplex Immunodetection

Maximize Targets, Minimize Cross-Reactivity

We offer in-house–produced and thoroughly tested antibodies conjugated to a variety of fluorophores, as well as extracellular-epitope reagents optimized for multiplex immunofluorescence, immunocolocalization, and high-parameter imaging. Guinea pig–derived antibodies are also available, providing additional species flexibility to help researchers build highly specific panels with minimal cross-reactivity. All antibodies are rigorously validated for applications including immunohistochemistry (IHC), immunocytochemistry (ICC), western blot (WB), and flow cytometry.

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FAQs

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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322 Results
  • Guinea Pig Anti-Alpha-Synuclein Antibody
    Guinea Pig Anti-Alpha-Synuclein Antibody
    A Guinea Pig Polyclonal Antibody to α-Synuclein
    • Product Code: APZ-035-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to α-Synuclein
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: α-Synuclein, Non-A4 component of amyloid precursor, Parkinson disease (autosomal dominant, Lewy body) 4, PARK1, PARK4, NACP, SNCA
  • Guinea pig Anti-Angiotensin-(1-7) Mas Receptor Antibody
    Guinea pig Anti-Angiotensin-(1-7) Mas Receptor Antibody
    A Guinea Pig Polyclonal Antibody to Angiotensin-(1-7) Mas Receptor
    • Product Code: AAR-013-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to Angiotensin-(1-7) Mas Receptor
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Proto-oncogene Mas, MAS1
  • Guinea Pig Anti-Aquaporin 4 (AQP4) (300-314) Antibody
    Guinea Pig Anti-Aquaporin 4 (AQP4) (300-314) Antibody
    A Guinea Pig Polyclonal Antibody to Aquaporin 4 (AQP4)
    • Product Code: AQP-014-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to Aquaporin 4 (AQP4)
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: WCH4, Mercurial-insensitive water channel, MIWC
  • Guinea pig Anti-ASIC1 Antibody
    Guinea pig Anti-ASIC1 Antibody
    A Guinea Pig Polyclonal Antibody to ASIC1 Channel
    • Product Code: ASC-014-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to ASIC1 Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Acid-sensing ion channel 1, Amiloride-sensitive brain sodium channel, BNaC2, ACCN2
  • Guinea pig Anti-ASIC3 Antibody
    Guinea pig Anti-ASIC3 Antibody
    A Guinea Pig Polyclonal Antibody to ASIC3 Channel
    • Product Code: ASC-018-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to ASIC3 Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Acid-sensing ion channel 3, Amiloride-sensitive cation channel 3, ACCN3, DRASIC
  • Guinea pig Anti-BDNF Antibody
    Guinea pig Anti-BDNF Antibody
    A Guinea Pig Polyclonal Antibody to BDNF
    • Product Code: ANT-010-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to BDNF
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Brain-derived neurotrophic factor
  • Guinea Pig Anti-Cannabinoid Receptor 1 (extracellular) Antibody
    Guinea Pig Anti-Cannabinoid Receptor 1 (extracellular) Antibody
    A Guinea Pig Polyclonal Antibody to Cannabinoid Receptor 1 (extracellular)
    • Product Code: ACR-001-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to Cannabinoid Receptor 1 (extracellular)
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: CB1, CB-R, CB1R, CANN6, Brain-type cannabinoid receptor, Central cannabinoid receptor, CNR1
  • Guinea pig Anti-Ca<sub>V</sub>1.2 (CACNA1C) Antibody
    Guinea pig Anti-CaV1.2 (CACNA1C) Antibody
    A Guinea Pig Polyclonal Antibody to CaV1.2 (CACNA1C) Channel
    • Product Code: ACC-003-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to CaV1.2 (CACNA1C) Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Voltage-dependent L-type calcium channel subunit α1C
  • Guinea pig Anti-Ca<sub>V</sub>1.3 (CACNA1D) Antibody
    Guinea pig Anti-CaV1.3 (CACNA1D) Antibody
    A Guinea Pig Polyclonal Antibody to CaV1.3 (CACNA1D) Channel
    • Product Code: ACC-005-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to CaV1.3 (CACNA1D) Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Voltage-dependent L-type calcium channel subunit α1D
  • Guinea Pig Anti-CHRM3 Antibody
    Guinea Pig Anti-CHRM3 Antibody
    A Guinea Pig Polyclonal Antibody to Acetylcholine receptor muscarinic 3
    • Product Code: AMR-006-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to Acetylcholine receptor muscarinic 3
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Cholinergic receptor muscarinic 3, Acetylcholine receptor muscarinic 3, M3 AChR
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